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Updated: Jan 18, 2026

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
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通过PafA介导的近距离标记辅助的敏感和特定亲和力净化质谱

Shihan Luo1, Lijuan Xie2, Lin Yang3

  • 1Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China; Lingang Laboratory, Shanghai 201306, China.

Cell reports methods
|September 9, 2025
PubMed
概括

一种新的蛋白质组学方法,APPLE-MS,增强了蛋白质与蛋白质相互作用 (PPI) 的检测,特别是在弱和膜相关相互作用方面. 这一进步改善了用于生物发现和治疗向的互动组映射.

关键词:
在AP-MS中使用AP-MS.科普:分子生物学互动的作用组.膜蛋白质组中的蛋白质组.蛋白质与蛋白质的相互作用接近性标签的标签.

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Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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