相关实验视频
Updated: Jan 18, 2026

12:10
Measuring Fast Calcium Fluxes in Cardiomyocytes
Published on: November 29, 2011
15.8K
Ca2+ 通过膜接触点流动
Lucia Barazzuol1, Marisa Brini2,3, Tito Calì4,3,5
1Department of Biomedical Sciences (DSB), University of Padova, Padova 35131, Italy.
Cold Spring Harbor perspectives in biology
|September 9, 2025
概括
离子 (Ca2+) 是细胞的重要信使. 膜接触部位 (MCS) 促进细胞器之间的Ca2+转移,但研究较少的部位的机制需要进一步研究以获得治疗见解.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
- 分子信号传输的方法
背景情况:
- 离子 (Ca2+) 在细胞代谢,信号传递和亡等过程中起到关键的第二信使作用.
- 膜接触点 (MCS) 是细胞区间之间快速和局部的Ca2+交换的关键接口.
- 虽然ER-线粒体和ER-PM接触得到了很好的研究,但涉及戈尔吉,溶解体,过氧体和核的MCS却不太了解.
研究的目的:
- 在各种MCS中提供Ca2+信号的全面审查.
- 突出研究不足的有机体在细胞内Ca2+信号传递中的作用.
- 强调需要对MCS中Ca2+转移的分子机制进行进一步研究,以获得潜在的治疗应用.
主要方法:
- 关于Ca2+信号和MCSs现有研究的文献综述.
- 对专注于Ca2+恒温和转移机制的研究进行分析.
- 综合了有关表征良好和研究不足的有机体接触的信息.
主要成果:
- 2+信号传递受到MCS的显著影响,超出了众所周知的ER-线粒体和ER-PM接口.
- 没有研究过的有机体,如戈尔吉,溶解体,过氧体和核,也通过它们的MCS参与Ca2+稳态.
- 在许多MCS中,控制Ca2+转移的分子参与者和调节机制在很大程度上仍未被阐明.
结论:
- 了解不同MCS中的Ca2+动态对于理解细胞信号传输至关重要.
- 需要对未经研究的MCS中Ca2+转移的分子基础进行进一步的研究.
- 阐明这些机制可能会揭示涉及Ca2+失调的疾病的新疗法标.
相关概念视频
Contact-dependent Signaling
46.8K
Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
Gap Junctions
In animal cells, gap junctions are formed...
46.8K
Membrane Fluidity
14.5K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
14.5K
Membrane Asymmetry Regulating Transporters
6.9K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
6.9K
Feedback Regulation of Calcium Concentration
3.9K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.9K
SNAREs and Membrane Fusion
12.4K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
12.4K
Intracellular Signaling Affects Focal Adhesions
3.5K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
3.5K

