在甲状腺癌中,CircPSD3通过调节SUCLG2来维持TCA循环和线粒体功能,从而加剧瘤的进展
Yijia Sun1, Beinan Han2, Jiawei Ge1
1Department of Endocrinology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, 510080, China.
Cell death & disease
|September 9, 2025
概括
像circPSD3这样的循环RNA越来越多地与甲状腺癌 (TC) 的进展有关. 这项研究揭示了circPSD3通过破坏三碳酸循环和线粒体功能来促进TC生长,提供了一个潜在的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 在全球范围内,甲状腺癌 (TC) 的发病率正在上升.
- 循环RNAs (circRNAs) 正在成为各种癌症的关键调节者,包括TC.
- 了解TC代谢中的circRNA作用对于确定新的治疗点至关重要.
研究的目的:
- 研究 circRNAs 在甲状腺癌代谢中的调节作用.
- 确定涉及TC进展的特定circRNA及其潜在机制.
- 探索TC的潜在基于circRNA的治疗策略.
主要方法:
- RNA测序以识别TC组织中差异表达的circRNA.
- 在体外和体内实验,以评估circPSD3对TC细胞的功能影响.
- 蛋白质学和代谢学,以阐明由circPSD3.3调节的分子通路.
- 分析circPSD3/miR-338-5p/SUCLG2轴及其对三碳酸 (TCA) 循环和线粒体功能的影响.
主要成果:
- 在TC组织中发现了circPSD3的高表达,与较大的瘤大小相关.
- circPSD3显著促进了TC细胞的增殖,并在体外和体内抑制了细胞灭亡.
- circPSD3作为miR-338-5p的分子海绵,对TCA循环中的关键酶SUCLG2进行上调.
- 敲除circPSD3损害了线粒体功能,减少了膜潜力和有氧呼吸,从而抑制了增殖并诱导了亡.
结论:
- circPSD3通过调节三酸循环和线粒体功能,在促进甲状腺癌进展方面发挥着至关重要的作用.
- 这种circPSD3/miR-338-5p/SUCLG2通路代表了一种推动TC发展的新机制.
- circPSD3是甲状腺癌的潜在诊断生物标志物和治疗点.
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