从一种新的内源性Trichoderma afroharzianum菌株AUMC 16,433中分离出的雌激酶的生产,优化和表征及其在染料脱色中的应用
Yehia A-G Mahmoud1, Nisrin S Alamin2, Tarek M Mohamed2
1Botany and Microbiology Department, Faculty of Science, Tanta University, Tanta, 31257, Egypt.
Microbial cell factories
|September 9, 2025
概括
来自内菌Trichoderma afroharzianum的一种新型雌激酶可以有效地去色合成染料. 这种酶为织行业的废水处理和环境修复提供了可持续的解决方案.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
- 酶工程是什么? 酶工程是什么?
背景情况:
- 织废水中的合成染料对环境和健康构成重大风险.
- 开发用于染料脱色的环保方法对于可持续的工业实践至关重要.
研究的目的:
- 为了隔离和表征一种来自内菌的新型雌激酶,用于合成染料脱色.
- 为了优化这种雌激酶的生产和净化.
- 评估其在降解各种工业染料方面的有效性.
主要方法:
- 使用ITS-rRNA测序对内菌进行隔离和鉴定.
- 使用普拉克特-伯曼设计和响应表面方法 (RSM) 优化雌激酶的生产.
- 通过硫酸沉和凝过来净化酶,然后通过SDS-PAGE来确定分子量.
- 酶的表征包括最佳温度,pH值和运动参数 (Vmax,Km).
- 对各种合成染料的染料脱色效率的评估.
主要成果:
- 鉴定出Trichoderma afroharzianum AUMC16433是一种新型的雌激酶生产物.
- 优化条件显著增强了酶的产生.
- 纯化雌激酶 (43 kDa) 在40°C和pH8.8时表现出最佳活性.
- 该酶在24小时内显示了马拉绿 (66%),甲醇蓝 (65.5%) 和素 (65.3%) 的高脱色率.
- 对于水晶紫色 (57.1%) 和甲基红色 (43.1%) 也观察到显著的色变.
结论:
- 来自Trichoderma afroharzianum的新型雌激酶表现出强大的染料脱色能力.
- 这种酶为处理织废水提供了一个有希望的,可持续的生物催化剂.
- 这些发现支持使用内菌作为新生物修复剂的来源.
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