通过指尖通过气相染色学/质谱学与固相微提取相结合,通过指尖获取单滴血液中的酒精度的确定
Koichi Saito1, Tomonori Asanuma1, Rie Ito1
1Department of Analytical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 2-4-41, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Legal medicine (Tokyo, Japan)
|September 10, 2025
概括
本研究提出了一种新的,低复杂度的方法,使用头空间固相微提取 (HS-SPME) 来测量血液中酒精度 (BAC). 该技术需要最少的血液样本,并准确地跟踪随时间的BAC变化.
科学领域:
- 分析化学 分析化学
- 法医毒理学 法医毒理学
背景情况:
- 精确的血中酒精度 (BAC) 测量对于临床和法医应用至关重要.
- 传统的方法通常需要大量的血液,这对受试者来说是一种负担.
- 开发最少侵入性和高效的BAC分析技术是一个持续的需求.
研究的目的:
- 开发一种低复杂度,准确的方法来测量血液中酒精度 (BAC),使用最少的血液样本.
- 为了适应头部空间固相微提取 (HS-SPME) 与气体染色学/质谱学 (GC/MS) 结合用于BAC分析.
- 评估该方法在监测 BAC 随时间变化和唾液等替代矩阵中的适用性.
主要方法:
- 使用头部空间固体相微提取 (HS-SPME) 采用聚乙烯糖醇 (PEG) 纤维.
- 采用气相色谱 (GC) 与DB-WAX毛细体柱和质谱 (MS) 检测.
- 分析了50μL的血液样本,使用20%的水性化溶液和70°C的头部空间提取20分钟.
主要成果:
- 在全血中,在100μg/mL时达到71.1%的乙醇回收率,在1000μg/mL时达到95.1%.
- 在人类志愿者饮酒后的BAC下降的准确跟踪,与Widmark方法相关.
- 证实了该方法对唾液样本的潜在适用性,以便更容易,非侵入性收集.
结论:
- HS-SPME-GC/MS提供了一种简化和准确的方法,用于减少样本体积的BAC测量.
- 开发的方法有效监测人类的BAC动力学,并显示了唾液分析的前景.
- 这种技术尽量减少受试者的负担,使其成为BAC评估的可行替代方案.
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