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母亲吸烟对胎盘DNA甲基化的直接和持久影响:复制和发现研究

Chloé Masdoumier¹, Lucile Broséus¹, Florent Chuffart²

¹Université Grenoble Alpes, INSERM U1209, CNRS UMR 5309, Institut pour l'Avancée des Biosciences (IAB), Team of Environmental Epidemiology Applied to Development and Respiratory Health, 38000 Grenoble, France.

Environmental epigenetics

|September 10, 2025

概括

这项研究复制了胎盘中母亲吸烟相关的DNA甲基化变化,发现大多数影响是暂时的,支持怀孕期间戒烟. 持续的表观遗传记忆效应不那么一致.

科学领域:

表观遗传学和环境健康
生殖流行病学生殖流行病学
基因组生物标志物基因组生物标志物

背景情况:

全表观基因组关联研究 (EWAS) 越来越多地将烟草吸烟与DNA甲基化变化联系起来.
对吸烟相关甲基化的复制研究很少,特别是在胎盘组织中.
了解产前暴露于烟雾的表观遗传影响对于公共卫生至关重要.

研究的目的:

使用EPIC阵列,复制母亲吸烟相关的CpG (细胞氨酸素) 和胎盘DNA甲基化区域.
在EPIC特定的CpG中探索与吸烟相关的新型甲基化模式.
将甲基化变化分类为暂时或持久,表明表观遗传记忆.

主要方法:

利用EDEN队列中的341个胎盘 (吸烟者,非吸烟者,前吸烟者) 的DNA甲基化数据.
采用候选方法来复制从450K阵列研究中先前识别的CpG和区域.
进行了探索性分析,以发现EPIC特定CpG中的新关联.

主要成果:

成功复制了38%的候选探头和9%的区域,证实了产前烟雾暴露对胎盘甲基化的影响.
确定了733个新的探测器和75个与吸烟相关的新区域,其中大多数影响是暂时的 (目前的吸烟者).
最多复制的命中是LEKR1位点;持久性CpG (前吸烟者) 是稀缺且不一致的.

结论:

大多数观察到的DNA甲基化变化仅与子宫内暴露有关,加强了怀孕期间戒烟的好处.
这项研究强调了许多吸烟诱导的甲基化变化的短暂性质,对持续的表观遗传记忆的证据有限.
强调可重复的OMIC研究的重要性,以牢固地了解环境暴露对健康生物标志物的影响.

关键词:

通过DNA甲基化.全表观基因组协会研究研究.持续的持续的持续的持续胎盘胎盘胎盘胎盘怀孕怀孕怀孕怀孕怀孕复制复制复制复制复制复制复制强度坚固性坚固性烟草吸烟吸烟烟草吸烟

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