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视觉化NEK9在行动:基于aptamer的光探针用于实时活细胞成像

Liyu Zhang¹, Ying Yang¹, Lidangzhi Mo¹

¹Shaanxi Institute for Pediatric Diseases, Xi'an Children's Hospital, Xi'an, China.

Nucleosides, nucleotides & nucleic acids

|September 10, 2025

概括

研究人员开发了一种新的基于aptamer的光探针,用于对NEK9激酶的活细胞成像. 这种工具克服了传统方法的局限性,使NEK9动态能够清晰可视化,细胞毒性最小.

科学领域:

分子生物学分子生物学
生物化学生物化学
细胞生物学细胞生物学

背景情况:

活细胞成像对于理解生物研究和医学中的蛋白质动力学至关重要.
传统的标签技术面临着诸如探针透性和细胞毒性差等挑战,阻碍了有效的活细胞成像.

研究的目的:

开发和验证一种基于aptamer的新型光探针,用于实时成像活细胞中的NEK9激酶.
克服现有的活细胞成像方法的局限性.

主要方法:

通过采用in vitro捕获-SELEX.识别了一种选择性地结合NEK9的DNA吸收体 (Apt-011),该吸收体使用in vitro捕获-SELEX.
设计了一种基于aptamer的'信号启动'光探针,利用aptamer诱导的形状变化来分离光灭器对.
验证了探头在活细胞成像中的性能,评估了NEK9可视化和细胞毒性.

主要成果:

成功识别了Apt-011,一种具有高特异性NEK9.9的DNA吸收体.
开发了一种基于aptamer的光探测平台,用于NEK9成像.
在活细胞中成功可视化细胞内NEK9,细胞毒性最小 (细胞活力>95%).

结论:

开发的以aptamer为基础的光探针为研究NEK9激酶及其相关信号通路提供了强大的工具.
这项工作建立了一个可概括的策略,通过合理的aptamer工程来克服活细胞成像中的关键瓶.

关键词:

亚普特美尔 (Aptamer) 是一种药物.在NEK9中,NEK9是NEK9.捕获-SELEXEX可以捕获.光探测平台是一个光探测平台.活细胞成像技术使用.

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