类似尼辛的生物合成基因集群广泛分布在微生物群中
David Hourigan1,2, Des Field1,2, Ellen Murray1,2
1APC Microbiome Ireland, Biosciences Institute, Biosciences Research Institute, University College, Cork, Ireland.
mBio
|September 10, 2025
概括
尼辛是一种强大的抗微生物,比以前想象的更广泛和多样化,在全球微生物组中发现了100多种新型变体. 这些发现突显了尼辛的存在.
科学领域:
- 微生物学和分子生物学
- 抗微生物是一种抗菌.
- 基因组学和生物信息学
背景情况:
- 细菌素,像尼一样,是具有治疗潜力的强效抗微生物药物.
- 尼辛对肠道微生物群的影响是已知的,但其自然丰富性,变种多样性和抗菌作用力仍未得到充分研究.
- 了解尼的患病率对于其作为生物治疗剂的应用至关重要.
研究的目的:
- 调查全球微生物组中尼生物合成基因集群 (nBGCs) 的流行和多样性.
- 识别新型尼变体并评估它们的抗菌活性.
- 探索nBGCs水平基因转移和适应的潜力.
主要方法:
- 对公开可用的基因组数据集进行分析,以确定尼辛生物合成基因集群 (nBGCs).
- 在移动遗传元素上对nBGC位置的生物信息预测.
- 新型尼变种的异质表达和抗菌活性测试.
主要成果:
- 识别了广泛和多样化的nBGCs,其中30%位于移动遗传元素上,包括致病细菌.
- 发现了细菌属之间nBGCs水平基因转移的证据.
- 描述了107种新的尼类,其中5种在异质表达时表现出抗菌活性. 具有新型链区域的Nisin VP的特征.
结论:
- 类似尼的基因群分布在全球范围,并且高度多样化,这表明它在微生物竞争中起着重要作用.
- 发现了许多具有抗微生物活性的新型尼变体,强调了它们的生物治疗潜力.
- 横向基因转移有助于在不同细菌物种中传播和适应尼生产能力.
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