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分析PET-MPs对男性不孕症的毒理学影响:从网络毒理学,孟德尔随机化和转录组学的见解

Yang Yang¹, Linfeng Li², Ning Zhang³

¹Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China; Engineering Research Center of Biopreservation and Artificial Organs, Ministry of Education, No 218 Jixi Road, Hefei Anhui230022, China; Key Laboratory of Population Health Across Life Cycle (Anhui Medical University)，Ministry of Education of the People's Republic of China, No 81 Meishan Road, Hefei, Anhui 230032, China; First School of Clinical Medicine, Anhui Medical University, Hefei 230032, China.

Reproductive biology

|September 10, 2025

概括

聚乙烯四甲微塑料 (PET-MPs) 可能会损害男性生育能力. 这项研究将CLK4确定为一个关键目标,将PET-MP与改变的T细胞透和破坏精子发育联系起来,影响男性生殖健康.

科学领域:

生殖生物学生殖生物学
环境毒理学环境毒理学
分子机制的分子机制

背景情况:

聚乙烯二甲微塑料 (PET-MPs) 越来越多地被认为是环境污染物.
新出现的证据表明,PET-MPs可能会对男性生殖功能产生负面影响.
调解这种损伤的精确分子途径在很大程度上仍未被阐明.

研究的目的:

研究PET-MPs诱导男性不孕症的分子机制.
为了确定涉及男性生殖功能障碍的PET-MPs的潜在基因标.
探索CLK4在PET-MP相关的男性不孕症中的作用.

主要方法:

生物信息分析包括瑞士目标预测,GWAS和eQTLGen数据库.
用SMR分析来确定基因与男性不孕症之间的因果关系.
大量和单细胞RNA测序 (scRNA-seq) 用于分析基因表达.
分子对接和动力学模拟以评估PET-MP-CLK4相互作用.

主要成果:

确定CLK4是PET-MPs的功能点,有助于男性不孕症.
增加的CLK4表达与增加的Th1细胞透和男性不孕症风险有关.
PET-MPs与CLK4结合,破坏精子生成并调节T细胞反应.
在精子分化过程中,CLK4表现出双相表达模式.

结论:

CLK4是一种潜在的分子标,可以调解PET-MPs对男性生育能力的不良影响.
通过准CLK4,PET-MPs可能会导致男性不育,影响T细胞透和精子生成过程.
了解CLK4介导的途径,可以了解环境对男性生殖健康的影响.

关键词:

环境污染物环境污染物男人不孕不育的情况网络毒理学网络毒理学更多关于PET-MPs 的文章

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