通过Src/PI3K信号通路对DBP诱导的精子生成损伤产生人参化物Rg3的保护作用
Huan Li1, Hongyan Wang1, Xiaolei Xu1
1School of Public Health, Beihua University, Jilin 132013, China.
Reproductive toxicology (Elmsford, N.Y.)
|September 10, 2025
概括
银化物Rg3通过Src/PI3K/Akt通路恢复精子健康和血屏障,保护男性的生育能力免受Di-n-butyl甲酸等环境毒素的影响.
科学领域:
- 生殖毒理学 生殖毒理学
- 分子生物学分子生物学
- 自然产品化学 自然产品化学
背景情况:
- 丁甲酸 (DBP) 是一种与男性生殖功能障碍相关的环境毒素.
- 甲酸暴露可以破坏丸结构,精子参数和血液丸屏障 (BTB).
- Src/PI3K/Akt信号通路在维持丸功能和BTB完整性方面发挥着至关重要的作用.
研究的目的:
- 阐明金色化物Rg3 (Rg3) 对DBP诱导的精子发生损伤的保护机制.
- 研究Src/PI3K/Akt路径在Rg3的保护作用中的作用.
- 评估Rg3对氧化应激和BTB相关蛋白质的影响.
主要方法:
- 在体内对暴露于DBP并接受Rg3.3治疗的小鼠进行了体内研究.
- 在体外研究中,使用TM4 Sertoli细胞治疗了单丁甲酸盐 (MBP) 和Rg3.
- 使用PP2和LY294002.2,对Src/PI3K通路进行药理抑制.
- 对丸结构,精子参数,蛋白质表达 (Cx43,p-Src,p-PI3K,p-Akt) 和氧化应激标志物 (MDA) 的评估.
主要成果:
- 在暴露于DBP的小鼠中,Rg3治疗恢复了丸结构,改善了精子数和运动性.
- 在体外和体外模型中,Rg3提高了Src,PI3K和Akt的酸化,并增强了连接素43 (Cx43) 的表达.
- Rg3增加了抗氧化酶活性,并降低了甲酸 (MDA) 水平,减轻了氧化应激.
- 抑制剂实验证实,Src/PI3K/Akt通路调解Rg3的保护作用.
结论:
- 银化物Rg3减轻了DBP/MBP诱导的男性生殖毒性.
- 通过增强抗氧化能力和重新激活Src/PI3K/Akt信号通路,Rg3可以防止精子受损.
- 通过Cx43稳定,Rg3恢复了BTB的完整性,这表明它有可能作为环境毒素诱导的男性不孕症的治疗剂.
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