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在Daphnia magna中受到Pyridaben诱导的生化和分子应激

Gülüzar Atli¹, Yusuf Sevgiler², Serdar Kilercioglu³

¹Çukurova University, Vocational School of İmamoğlu, Adana, Turkey; Çukurova University, Biotechnology Research and Application Center, Adana, Turkey.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP

|September 10, 2025

概括

这项研究揭示了pyridaben (PDB) 通过破坏氧化应激和透调节,导致大夫尼亚大的亚急性毒性. 综合生物标记分析显示了显著的分子和生化变化,突出了生态风险.

科学领域:

环境毒理学环境毒理学
水生生态毒理学水生生态毒理学
生物标志物研究的研究.

背景情况:

皮里达本 (PDB) 是一种对水生生物有毒的有力虫杀菌剂,抑制线粒体复合体I.
了解水生生物中的亚急性毒性机制对于生态风险评估至关重要.

研究的目的:

通过使用综合分子和生化多生物标志物方法,评估大夫尼亚大中PDB的亚急性毒性.
评估对PDB暴露的氧化应激和度调节反应.
应用综合生物标志物响应 (IBR) 指数进行综合性毒性评估.

主要方法:

达芬尼亚大暴露在PDB (0.20-0.80TU) 的不同度下.
对抗氧化酶 (SOD,GPX,CAT,GST),GSH,TBARS,Ca2+-ATPase和总蛋白质的生化分析.
对基因表达和微RNA (miR-153) 水平的分子分析.
综合生物标志物响应 (IBR) 指数的计算.

主要成果:

暴露于PDB改变了抗氧化剂动态,增加了CAT,GST,GSH和TBARS,同时减少了SOD和GPX.
与抗氧化酶相关的Ca2+-ATPase活性升高,表明适应性透调节.
观察到氧化应激基因的下调和miR-153的上调.
IBR分析显示,从0.35TU开始的显著反应,在0.50TU及以上的最大效应.

结论:

PDB诱导了Daphnia magna中显著的相互关联的生化和分子变化.
包括IBR在内的多生物标记方法对于评估PDB的生态风险是有效的.
这些发现阐明了水生生物对杀性压力的适应性反应的分子途径.

关键词:

酸2+) 亚特酶氧化应激是一种氧化应激.在Pyridaben中,我们可以看到Pyridaben.与压力相关的基因表达.在 miR1533 中使用.

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