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微生物组和挥发性有机化合物反映了室内环境中的分解状态

Veronica M Cappas¹, Reena Roy¹, Emily R Davenport²

¹Forensic Science Program, Eberly College of Science, The Pennsylvania State University, University Park, PA, United States.

Science & justice : journal of the Forensic Science Society

|September 10, 2025

概括

这项研究探讨了微生物组和挥发性有机化合物 (VOC) 在分解过程中如何发生变化. 鼻孔被证明是最好的预测分解阶段,揭示特定细菌和VOC之间的联系.

科学领域:

法医科学法医科学法医科学
微生物学微生物学
分析化学分析化学

背景情况:

鉴于分解的可变性,确定死后间隔 (PMI) 是具有挑战性的.
微生物活动驱动分解并产生挥发性有机化合物 (VOC).
微生物群落和VOC都是PMI估计的潜在指标.

研究的目的:

调查猪分解过程中微生物组和VOC档案的时间变化.
探索微生物群落,VOC和分解阶段之间的关系.
为了确定最佳的采样地点和微生物/VOC标志物用于PMI的确定.

主要方法:

在受控的室内环境中,猪尸被用作人类同类物.
随着时间的推移,从多个位置 (腹部,门,耳朵,鼻孔) 取样的微生物群落.
使用头部空间采样分析的挥发性有机化合物 (VOCs).
采用了统计分析,包括随机森林和线性回归模型.

主要成果:

在整个分解过程中观察到显著的细菌群体转移 (p < 0.001),因采样地点而异.
摩拉克塞拉属,普拉诺可可属,乳酸菌属和斯塔菲洛可可属家族影响了社区的动态.
鼻孔采样地点被确定为预测分解阶段最有效的地点.
单个VOC显示了实质性的时间波动,而功能组显示了轻微的变化.
在特定的细菌 (Planococcaceae,Tissierellaceae) 和腹部区域的内醇VOC之间发现的相关性.

结论:

在分解过程中,微生物组和VOC档案可预测地演变.
鼻孔为基于微生物组的PMI估计提供了一个有希望的位置.
这项研究支持了微生物活动,VOC产生和分解进展之间的联系.

关键词:

社区转移社区转移分解分解是指分解.微生物组是一个微生物组.验尸后的时间间隔挥发性有机化合物挥发性有机化合物

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