轴膜蛋白CFAP91的近距离标记识别了EFCAB5,该蛋白调节精子运动
Haoting Wang1,2, Keisuke Shimada1,3, Anh Hoang Pham1,2
1Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.
Nature communications
|September 10, 2025
概括
CFAP91对于精子鞭状体的形成和男性生育能力至关重要,由脚手架辐射螺杆3 (RS3) 组装. 精子特异性蛋白质EFCAB5也调节精子的运动和生育能力.
科学领域:
- 细胞生物学 细胞生物学
- 生殖生物学 生殖生物学
- 结构生物学 结构生物学
背景情况:
- 辐射射线 (RSs) 对乳毛和精子鞭毛的运动至关重要.
- 一种RS3蛋白CFAP91与男性不孕症有关,但其功能尚不清楚.
研究的目的:
- 阐明CFAP91在精子鞭子形成和男性生育能力中的分子功能.
- 确定CFAP91相互作用蛋白及其在精子结构和功能中的作用.
主要方法:
- 使用了Cfap91淘汰赛 (KO) 鼠标模型.
- 使用转基因救援模型,标记为CFAP91.1.
- 在精子中进行免疫沉和近距离标记 (BioID2).
主要成果:
- Cfap91 KO小鼠显示精子鞭毛形成受损和不孕.
- CFAP91与RS3蛋白CFAP251和LRRC23相互作用,这对RS3局部化至关重要.
- EFCAB5被确定为精子特异的CFAP91-近位成分,其KO降低了精子的运动和生育能力.
结论:
- CFAP91对于RS3复合体组装和精子发育至关重要.
- EFCAB5作为精子运动的精子专门调节器.
- 这些发现提升了对哺乳动物精子和男性不孕症的轴突膜专业化的理解.
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