能量不足选择拥挤的活上皮细胞进行挤出
Saranne J Mitchell1,2,3,4, Carlos Pardo-Pastor1,2,4,5, Anastassia Tchoumakova1,2,4
1The Randall Centre for Cell & Molecular Biophysics, School of Basic & Medical Biosciences, King's College London, London, UK.
Nature
|September 10, 2025
概括
用于挤压的低能量的上皮细胞被选择. 通过表皮通道 (ENaC) 进入引发了这一过程,突出显示了离子通道在细胞循环中的作用.
科学领域:
- 细胞生物学
- 生理学
- 生物物理
背景情况:
- 皮质细胞通过快速的细胞分裂和死亡形成一种保护性屏障.
- 细胞分裂和死亡的不平衡会导致屏障功能障碍或瘤形成.
- 由Piezo1介导的机械力量影响细胞分裂和挤压诱导的细胞挤出.
研究的目的:
- 确定选择哪个拥挤的细胞进行挤出的特定机制.
- 阐明细胞能量和膜潜力的作用.
- 了解皮埃佐1在上皮细胞循环中的上游调节者.
主要方法:
- 研究细胞对表皮细胞拥挤的反应.
- 测量细胞能量 (ATP水平) 和膜潜力.
- 分析的离子通道活动,包括表皮道 (ENaC),Kv1.1,Kv1.2,和SWELL1.
- 观察到的水运输和细胞体积变化.
主要成果:
- 拥挤的细胞具有最低的能量和膜潜力,优先选择用于挤出.
- 通过ENaC诱导进入,导致细胞脱极化.
- 具有有限ATP的细胞不能再极化并经历水的退出,导致缩小和激活挤出.
- 这种机制在Piezo1的上游作用,涉及ENaC作为张力传感器.
结论:
- 细胞能量水平和膜潜力是细胞挤出的关键决定因素.
- ENaC充当传感器,识别用于挤压的低能电池并防止Piezo1的不适当激活.
- 水调节和特定的离子通道是表皮细胞循环和屏障完整性的关键调节者.
相关概念视频
Microvilli
8.9K
Microvilli are tiny finger-like projections found on the surface of certain cells. Their purpose is to increase the surface area of the cell's apical surface, resulting in more effective absorption or secretion of substances.
These microvilli are predominantly present in cells lining the small intestine, kidney tubules, and certain cells in the respiratory and reproductive systems. By significantly expanding the surface area of the cell membrane, microvilli enhance the cell's capacity...
These microvilli are predominantly present in cells lining the small intestine, kidney tubules, and certain cells in the respiratory and reproductive systems. By significantly expanding the surface area of the cell membrane, microvilli enhance the cell's capacity...
8.9K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.6K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.6K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.4K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.4K


