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贝贝西亚比格米纳乳酸脱酶的表达,净化和生物化学特征

Safiye Merve Bostancioglu¹, Ozkan Danis², Sinem Kocer³

¹Department of Biology, Faculty of Science, Marmara University, Göztepe, 34722, Istanbul, Türkiye.

Molecular biotechnology

|September 10, 2025

概括

这项研究的特征是乳酸脱酶 (LDH) 来自Babesia bigemina,一个寄生虫引起牛乳病. 该酶表现出稳定性和特定的运动性质,为新的治疗点提供了潜力.

科学领域:

生物化学生物化学
寄生虫学的寄生虫学
分子生物学分子生物学

背景情况:

贝贝西亚大虫 (Babesia bigemina) 引起牛乳病,这是一个重要的经济疾病.
乳酸脱酶 (LDH) 对于寄生虫的无氧糖解至关重要.

研究的目的:

为了克隆,表达和净化Babesia bigemina LDH.
用生化方法来描述酶的稳定性,动力学和最佳条件.

主要方法:

在大肠杆菌中基因克隆和基因表达.
使用亲和色谱进行净化.
生物化学测定稳定性,pH最佳值,热稳定性和运动参数.

主要成果:

纯化的B. bigemina LDH对酸盐 (Km = 0.2585 mM) 呈现了迈凯利斯-门动力学和对NADH的潜在性行为 (K0.5 = 0.3094 mM).
该酶在4°C下稳定9天,在pH值8.5.5时表现出最佳活性.
活动在30°C和40°C时保持不变.

结论:

描述为B. bigemina的LDH功能提供了洞察力.
这些发现可能有助于开发针对寄生虫的糖解路径的疗法.

关键词:

贝贝西亚大双胞胎生物化学表征生物化学表征酶动力学酶动力学乳酸脱酶的使用方法传播的原生动物.

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