基于ICP-MS的纳米粒子表征的进展:生物样本分析的技术和挑战
Filip Gregar1, Daniel Baron1, Tomáš Pluháček1
1Department of Analytical Chemistry, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic.
Journal of separation science
|September 10, 2025
概括
在生物系统中对工程纳米粒子 (NP) 的准确分析至关重要. 本综述强调了感应合等离子体质谱法 (ICP-MS) 方法的进展,包括单粒子ICP-MS和连线技术,用于检测和量化NP.
科学领域:
- 环境科学 环境科学
- 分析化学 分析化学
- 纳米技术纳米技术
背景情况:
- 工程纳米粒子 (NP) 越来越多地用于消费者和生物医学产品.
- 有关NP在生物系统中的积累,转化和毒性存在担忧.
- 准确的分析方法对于在复杂的生物矩阵中检测,描述和量化NP至关重要.
研究的目的:
- 批判性地评估最近 (从2020年1月起) 基于ICP-MS的方法在生物样本中进行NP分析的进展.
- 为了比较单颗粒ICP-MS (spICP-MS) 和连字符的ICP-MS技术.
- 讨论这些方法的优点,局限性和互补性,以了解NP命运.
主要方法:
- 对基于ICP-MS的NP分析方法的最新文献的综述.
- 单粒子ICP-MS (spICP-MS) 的详细检查,包括提取,粒子类型,矩阵和限制.
- 讨论与ICP-MS.相结合的带连线的技术 (例如染色学,电泳).
- 覆盖激光切除spICP-MS用于组织成像.
主要成果:
- spICP-MS是最广泛用于直接确定NP大小,度和金属含量的方法.
- 连接式技术为NP大小分布,聚合和相互作用提供了更深入的见解.
- 激光切除spICP-MS可实现空间分辨的NP检测和组织成像.
- 无论是spICP-MS还是连字符的方法都有特定的优势和局限性.
结论:
- 由于其灵敏度和定量能力,ICP-MS,特别是spICP-MS是生物样本中NP分析的领先技术.
- 带连线的ICP-MS技术补充了spICP-MS,提供了关于NP行为和相互作用的额外信息.
- 结合使用单粒子和连字符的ICP-MS方法,可以更全面地了解生物系统中的NP命运.
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