伪桑托蒙纳斯 (Pseudoxanthomonas taiwanensis) 和伪蒙纳斯 (Pseudomonas thermotolerans):在固态发酵中的新型热友氨酶生产者
Anna Taulé1, Teresa Gea1, Alejandra Cerda1
1GICOM Research Group, Department of Chemical, Biological and Environmental Engineering School of Engineering, Building Q Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
Biotechnology and bioengineering
|September 11, 2025
概括
使用本地微生物进行非无菌固态发酵,可以有效地从土豆皮中产生粉酶. 这种方法超越了注射方法,突出了土著热友细菌在工业酶生产中的潜力.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
- 酶技术 酶技术是一种
背景情况:
- 热稳固的粉酶对于工业生物处理至关重要.
- 固态发酵 (SSF) 为酶生产提供了一种可持续的方法.
- 马皮是一种丰富的,低成本的基质,用于微生物发酵.
研究的目的:
- 为了研究使用非无菌SSF与土豆皮的粉酶生产.
- 为了比较本土微生物种群与接种的Thermomyces lanuginosus.
- 为了识别和表征热友性氨酶产生菌株.
主要方法:
- 非无菌的土豆皮的固体发酵 (SSF).
- 非注射和注射 (Thermomyces lanuginosus) 批量反应堆的比较.
- 微生物选择的顺序批量反应器 (SBR) 策略.
- 隔离和鉴定热友细菌菌株.
主要成果:
- 非注射反应器的酶生产率 (3920 U/gDM/天) 比注射反应器 (1823 U/gDM/天) 高得多.
- 在SBR中没有检测到Thermomyces lanuginosus,并且在接种疫苗时没有观察到持续的生产力改善.
- 他们分离了三种热友的粉酶产生菌株:Bacillus coagulans,Pseudoxanthomonas taiwanensis和Pseudomonas thermotolerans. 这三种菌株分离出来.
结论:
- 使用本地微生物群落的非无菌SSF是一种高效和可扩展的粉酶生产方法.
- 这种方法支持循环生物经济原则,并减少对外部微生物注射剂的依赖.
- 据报道,Pseudoxanthomonas taiwanensis和Pseudomonas thermotolerans是粉酶的生产者,扩大了工业酶应用的工具包.
相关概念视频
Hyperthermophilic Bacteria
502
Domain Bacteria includes some unique hyperthermophilic species. They exhibit remarkable adaptations that enable survival in extreme environments.Thermotoga species are rod-shaped, gram-negative, non-sporulating hyperthermophiles that form a sheath-like envelope called a toga. They ferment sugars or starch, producing lactate, acetate, CO₂, and H₂, and can also grow via anaerobic respiration using H₂ and ferric iron. Found in hot springs and hydrothermal vents, over 20% of their...
502
Diversity of Archaea I
552
Archaea, a domain of single-celled microorganisms, are classified into five major phyla based on genetic and biochemical characteristics: Euryarchaeota, Crenarchaeota, Thaumarchaeota, Korarchaeota, and Nanoarchaeota. Among these, the phylum Euryarchaeota is notable for its remarkable diversity in morphology, metabolism, and ecological adaptations.Morphological and Metabolic DiversityMembers of Euryarchaeota exhibit a variety of cellular shapes, including rods and cocci. Their metabolic pathways...
552
Microbial Fermentation
1.4K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.4K
Diversity of Archaea IV
415
Hyperthermophilic archaea are a group of extremophiles thriving at temperatures above 80°C, often in hydrothermal vents and volcanic soils where conditions surpass the boiling point of water. At such temperatures, proteins, membranes, and DNA in most organisms degrade, but hyperthermophiles have evolved remarkable adaptations to maintain stability and function.Unique Cellular FeaturesHyperthermophilic membranes are composed of a monolayer of biphytanyl tetraether lipids, which resist...
415


