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相关概念视频

DNA Topoisomerases02:02

DNA Topoisomerases

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Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
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Conserved Binding Sites01:49

Conserved Binding Sites

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Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
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Protein Folding01:22

Protein Folding

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Overview
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相关实验视频

Updated: Jan 18, 2026

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
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Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

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大规模的形式分析解释了G-四重复拓景观.

Michał Jurkowski1, Mateusz Kogut1, Michał Olewniczak1

  • 1Department of Physical Chemistry, Gdańsk University of Technology, Narutowicza St 11/12, Gdańsk 80-233, Poland.

The journal of physical chemistry. B
|September 11, 2025
PubMed
概括

G-四重复 (G4) 可以形成26个拓,但只有14个被观察到. 长距离环和G4的螺旋性限制了G4的折叠,解释了观察到的模式并帮助G4结构设计.

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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

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Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
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Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
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科学领域:

  • * 分子生物学 * 分子生物学
  • * * 结构生物学 结构生物学
  • * 生物信息学是一门学科.

背景情况:

  • *G四重复体 (G4) 是四链DNA结构,对基因组稳定性和转录至关重要.
  • *G4s在人类基因组中丰富,在基因调节中起着至关重要的作用.
  • * 在26个理论G4折叠拓中,只有14个被实验观察到,这引发了关于可访问性和形成决定因素的问题.

研究的目的:

  • * 调查G4折叠拓的能量可访问性.
  • * 确定控制G4折叠模式的分子因素.
  • * 为观察到的G4拓景观提供几何解释.

主要方法:

  • *使用128个DNA序列对G4形态空间进行计算探索.
  • * 在形折叠过程中产生了近20,000个独特的G4形状.
  • * 在26个理论拓中对G4形状进行折叠性评估.

主要成果:

  • * 在26个理论G4拓中观察到可折叠性的显著差异.
  • * 12种拓中的长距离螺旋环施加了环长限制,阻碍了形成.
  • *G4螺旋体控制长距离螺旋圈的发生,影响右手和左手G4的折叠偏好.

结论:

  • * 该研究为G4折叠模式和拓景观提供了几何解释.
  • * 长距离环长度和G4螺旋性是G4折叠的关键决定因素.
  • * 结果为具有特定拓的G4结构的合理设计提供了洞察力.