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自启动复合体的进化多样化:降低了Atg101的依赖性和Atg9与Atg13结合的变化
Zefeng Lai1,2, Yutaro Hama1, Masahide Oku3
1Department of Biochemistry and Molecular Biology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Autophagy
|September 11, 2025
概括
自的启动涉及ULK/Atg1复合体,其成分因物种而异. 这项研究表明,在获得ATG29和ATG31之后,ATG101在一些真菌中消失,影响了ATG13-ATG9相互作用.
科学领域:
- * 分子生物学 * 分子生物学
- * 进化生物学 进化生物学
- * 细胞生物学 * 细胞生物学
背景情况:
- *宏自/自是一种涉及自细胞的保存细胞降解过程.
- *ULK/Atg1复合体通过支架ATG蛋白和ATG9囊泡启动自细胞形成.
- *哺乳动物ULK复合体 (ULK1/2,RB1CC1,ATG13,ATG101) 与酵母 (Atg1,Atg17,Atg13,Atg29,Atg31) 有所不同.
研究的目的:
- * 为了研究ULK/Atg1复杂组件的进化变化.
- * 了解某些真菌系中ATG101损失的功能影响.
主要方法:
- *在真核细胞群中进行BLAST分析,以确定基因存在/不存在.
- *酵母两种混合试验,以评估蛋白质相互作用 (ATG101,ATG13,ATG9).
- *对Komagataella phaffii*中的基因删除的功能分析.
主要成果:
- * *ATG101*在*ATG29*/*ATG31*收购后的某些Holomycota系中消失了;*ATG13上限结构收购之前的*ATG101*损失.
- *ATG101对于哺乳动物的ATG13-ATG9相互作用至关重要,但由于结合部位的改变,在Aspergillus oryzae中不可用.
- 在Komagataella phaffii,atg101和atg31中,删除对饥饿诱导的自产生添加效应,这两种蛋白质对于Atg1复合体组装至关重要.
结论:
- *ULK/Atg1复合体的进化涉及基因丢失和获取,适应不同的细胞需求.
- *减少对ATG101对ATG13-ATG9相互作用和Atg1复合组合的依赖,促进了其在某些真菌系中丧失.
- * 这些发现揭示了核心自机械的进化可塑性.
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