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Updated: Jan 18, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

对比转录基因分析揭示了Siniperca scherzeri中的关键生长相关基因和替代拼接事件

Hao Xu¹, Jian Jin¹, Yang Li¹

¹Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Jimei University, Xiamen, China.

Marine biotechnology (New York, N.Y.)

|September 11, 2025

概括

与生长缓慢的个体相比,快速生长的Siniperca scherzeri在基因表达和替代拼接方面表现出差异. 这些涉及转录和拼接调节的分子机制提高了这一重要的水产养殖物种的生长率.

科学领域:

水产养殖是水产养殖的一种方式.
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

西尼 (Siniperca scherzeri) 是中国一个重要的水产养殖物种,因其抗病能力而受到重视,但由于生长缓慢而受到限制.
了解生长变化的遗传基础对于提高水产养殖效率至关重要.

研究的目的:

研究分子机制,包括基因表达和替代拼接,区分快速生长的 (FG) 和缓慢生长的 (SG) S. scherzeri.
为了确定在S. scherzeri水产养殖中选择性繁殖的潜在分子标记物和基因.

主要方法:

用RNA测序 (RNA-seq) 来分析FG和SG S. scherzeri个体的肝脏组织.
进行了差异基因表达 (DEG) 和差异替代拼接 (DSG) 分析.
进行了基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径丰富分析.

主要成果:

在FG和SG组之间确定了875个DEG和622个DSG.
在骨发育,细胞外矩阵重塑,信号转导和细胞生长/亡中,DEGs得到了丰富.
DSG在RNA拼接,蛋白质降解,核糖体生物发生,肌肉结构和能量代谢途径方面显著丰富,FG表现出更多的替代拼接事件.

结论:

在S. scherzeri的生长速度差异很可能是由于在转录和拼接层面的协调调节.
替代拼接在提高生长率方面发挥着重要作用.
结果提供了对生长的分子基础和育种计划的资源的洞察.

关键词:

西尼佩尔卡是个开玩笑的人.另一个替代拼接.增长的增长增长的增长在RNA-seqqq.

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