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Updated: Jul 9, 2026

Determining Membrane Protein Topology Using Fluorescence Protease Protection FPP
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Determining Membrane Protein Topology Using Fluorescence Protease Protection FPP

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基于TurboID对有机细胞膜蛋白相互作用体的映射与数字-透化的映射

Yang Sun1,2, Lan Yang1, Jingzi Zhang3

  • 1Centre for Cellular Biology and Signaling, Zhejiang University-University of Edinburgh Institute, Haining 314400, Zhejiang, China.

Biophysics reports
|September 11, 2025
PubMed
概括

我们开发了一种结合TurboID和数字透的新方法,用于绘制有机细胞膜中的蛋白质相互作用. 这种技术减少了背景噪声,提高了用于细胞平衡研究的蛋白质组分析的精度.

关键词:
迪吉托尼-透性-通性-通性有机体互动体 (互动体)蛋白质蛋白质相互作用靠近标签的标签.在REEP1/REEP6中使用.

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Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...