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Updated: Jan 18, 2026

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从单个小鼠B细胞中快速生成抗原特异单克隆抗体

Xuanxiu Ren1, Yiwei Zhang1, Gan Zhang1

  • 1State Key Laboratory of Virology and Biosafety, College of Life Sciences, Wuhan University, Wuhan 430072, China.

Biophysics reports
|September 11, 2025
PubMed
概括

这项研究优化了一种用于从单抗原特异性小鼠B细胞中测序和克隆抗体基因的协议. 这种精细的方法增强了单克隆抗体的产生和功能查,以更快地发现.

关键词:
高通量抗体表达的高通量抗体表达.单克隆抗体是一种单克隆抗体.鼠标抗体 鼠标抗体单个B细胞的PCR.

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Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
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