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在Acropora cervicornis中映射热应激诱导的核心基因素翻译后修饰

Cassandra N Fuller¹, Sabrina Mansoor², Santiago J Guzman¹

¹Department of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, United States.

Environmental epigenetics

|September 11, 2025

概括

使用受困离子移动谱法 (TIMS) 和并行积累-序列碎片化 (PASEF) 开发了一种新型高度敏感的方法,用于分析基质子转化后修饰 (PTMs). 这项技术确定了珊瑚对热应激反应的关键PTM变化.

科学领域:

表观遗传学和分子生物学
海洋生物学和毒理学

背景情况:

基因组转化后修饰 (PTMs) 对于调节染色体结构和功能至关重要.
现有的分析方法难以区分组素PTM异构体和检测低丰度修饰.
了解珊瑚对热压力等环境压力的反应,需要对表观遗传变化的敏感检测.

研究的目的:

开发和验证一种高灵敏度的方法来分析质子PTMs.
应用这种方法来研究在急性热应激下珊瑚中的组素PTM变化.
为了确定与珊瑚热反应相关的特定表观遗传标记.

主要方法:

一种自下而上的蛋白质组学方法,结合了纳米液态染色学 (nLC),被困离子流动性谱学 (TIMS),数据依赖采集 (DDA),并行积累-串行碎片化 (PASEF) 和高分辨率飞行时间并列质谱学 (ToF-MS/MS).
使用受威胁的鹿角珊瑚物种*Acropora cervicornis*受到急性热应激的方法验证.
与传统的LC-MS/MS方法进行比较,以评估特异性,灵敏度和动态范围.

主要成果:

开发的TIMS-PASEFMS/MS方法表现出高的特异性,灵敏性和广泛的动态范围 (大小6级).
在*Acropora cervicornis*中,在热应力过程中发现了核心质子PTM的显著变化.
确定的特定PTM包括增加的H4乙化 (2ac,3ac) 和降低的H4K12ac,H4K16ac,H4K20me2,H2AK5ac,H2AK7ac,H2AK9ac,H2AK12ac,H2AK14ac和H2AK74ac.

结论:

蒂姆斯-帕塞夫MS/MS方法提供了一种强大的工具,用于对包括低丰度标志物在内的基因组PTM进行深入和可靠的分析.
这种方法可以识别珊瑚中关键的表观遗传变化,以应对热应激等环境挑战.
这些发现有助于理解珊瑚的弹性和它们对气候变化的反应背后的分子机制.

关键词:

它们是Acropora cervicornis,它们是Acropora.在 H2A 基因素中.在 H2B 基因组素中.在 H4 基因素中,nLC-TIMS-ddaPASEF-TOF MS/MS MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS 翻译后的修改翻译后的修改鹿角珊瑚是一种珊瑚.

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