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解读高和低发育能力的猪卵细胞的DNA甲基化和转录组概况的差异

Laura Abril-Parreño^1,2, Jordana S Lopes³, Jon Romero-Aguirregomezcorta^1,2

¹Physiology of Reproduction Group, Department of Physiology, Faculty of Veterinary Medicine, International Excellence Campus for Higher Education and Research (Campus Mare Nostrum), University of Murcia, 30100 Murcia, Spain.

Environmental epigenetics

|September 11, 2025

概括

在猪体内成熟 (IVM) 的结果与体内成熟相比,卵细胞的发育能力较低. 基因表达和DNA甲基化的差异,受捐赠者的年龄和成熟方法的影响,是这种能力差距的基础.

科学领域:

生殖生物学生殖生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
动物生物技术动物生物技术

背景情况:

试管成熟 (IVM) 对于动物的辅助生殖技术至关重要.
与体内成熟的卵细胞相比,IVM卵细胞的发育潜力往往降低.
这种发展能力差异的分子基础在很大程度上是未知的.

研究的目的:

调查猪卵细胞在体外和体内成熟的基因表达和DNA甲基化资料之间的差异.
确定有助于卵子细胞不同发育能力的分子机制.
为了比较来自青春期前母 (IVM) 和成年母猪 (体内成熟) 的卵子.

主要方法:

单细胞RNA测序用于分析基因表达.
单细胞双硫酸盐测序以评估DNA甲基化模式.
对卵细胞发育能力的比较分析 (分裂和胚胎细胞分裂率).

主要成果:

IVM卵细胞的裂变和胚胎细胞分裂率明显低于体内成熟的卵细胞.
与体内成熟的卵细胞相比,IVM卵细胞显示1297个下调和476个上调的差异表达基因 (DEGs).
虽然全球DNA甲基化情况相似,但在关键的基因组领域发现了特定的甲基化区域.

结论:

捐赠者的年龄和成熟条件显著影响卵细胞基因表达和发育能力.
表观遗传修饰,特别是DNA甲基化,在区分卵细胞发育潜力方面发挥着作用.
结果提供了关于改善IVM协议以提高胚胎生产的见解.

关键词:

细胞分裂是细胞的分裂.作为一个表观基因组,它是表观基因组.多个原子的分析.卵细胞成熟的过程猪类动物猪类动物

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