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甲酸暴露和潜在风险的表观遗传特征:使用Infinium MethylationEPIC BeadChip进行DNA甲基化分析

Ping-Hsun Wu^1,2,3, Shiau-Ching Chen⁴, Chun-Jui Chien⁴

¹Division of Nephrology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, Taiwan.

Environmental epigenetics

|September 11, 2025

概括

酸盐暴露会影响人类健康,改变DNA甲基化模式. 这项研究确定了与单乙烯基甲酸盐 (MEHP) 相关的特定甲基化标志物,提供了对毒素暴露和表观遗传调节的见解.

科学领域:

环境表观遗传学环境表观遗传学
毒理学毒理学毒理学
人类健康人类健康人类健康

背景情况:

酸盐是广泛存在的环境污染物,与不良健康结果有关,包括免疫功能障碍,发育毒性和荷尔蒙干扰.
表观遗传修饰,特别是DNA甲基化,对环境因素很敏感,可以作为暴露于有毒物质的早期生物标志物,而不会改变DNA序列.

研究的目的:

为了研究甲酸暴露对人类DNA甲基化的影响.
识别与酸盐暴露相关的潜在表观遗传标记物及其生物功能.

主要方法:

使用Illumina Infinium MethylationEPIC BeadChip阵列对389名参与者的DNA甲基化数据进行分析.
量化15种尿甲酸代谢物,以评估暴露水平.
鉴定和功能分析与甲酸代谢物相关的差异甲基化CpG位点 (DMC),特别是单乙烯基甲酸 (MEHP).

主要成果:

观察到甲酸盐代谢物度的性别特异性差异,女性显示的水平高于男性.
与MEHP暴露有关的53个差异甲基化CpG位点 (DMC) 被确定,其中11个始终被检测到.
功能性丰富分析表明,这些DMC参与了关键途径,包括蛋白质/核酸结合,免疫反应和离子通道调节.

结论:

这项研究确定了与酸盐相关的新型DNA甲基化标记物和相关基因.
这些发现提供了对底层甲酸盐毒性的表观遗传机制的宝贵见解.
这些甲基化标记物在监测环境中毒素暴露时具有临床应用的潜力.

关键词:

通过DNA甲基化.生物标志物生物标志物环境有毒毒剂环境有毒剂在表观基因组学上,表观基因组学.甲酸的使用方法

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