相关实验视频

Updated: Jul 11, 2026

Massively Parallel Reporter Assays in Cultured Mammalian Cells
11:03

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Published on: August 17, 2014

使用优化条形码报告器和自动计算管道的多重转录因子活动检测协议

Max Trauernicht1, Vinícius H Franceschini-Santos1, Hatice Yücel1

  • 1Oncode Institute, Division of Molecular Genetics, Netherlands Cancer Institute, 1066 CX Amsterdam, the Netherlands.

STAR protocols
|September 11, 2025
PubMed
概括

这项研究引入了一种新的方法,用于测量转录因子 (TF) 活动,使用条形码记者库. 这种高通量方法使得TF网络在单元格中的定量分析成为可能.

关键词:
生物信息学是一种生物信息学.细胞生物学 细胞生物学细胞培养培养的细胞培养.基因表达 基因表达基因组学就是基因组学.分子生物学分子生物学在RNAseqqq中使用.序列分析是指进行序列分析.序列化是指测序的使用.系统生物学 系统生物学

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Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...