podosome 底部的 myosins 1e/f 调节了 podosome 的动态,并促进了巨细胞的迁移
Pasquale Cervero1, Sarah R Barger2, Perrine Verdys3
1Institut für medizinische Mikrobiologie, Virologie und Hygiene, Universitätsklinikum Eppendorf, Martinistr. 52, Hamburg 20246, Germany.
European journal of cell biology
|September 11, 2025
概括
一类的myosins,Myo1e和Myo1f,将富含actin的podosomes与等离子膜连接起来. 它们的缺失改变了体结构和细胞迁移,揭示了新的体亚体结构:"基础".
科学领域:
- 细胞生物学 细胞生物学
- 细胞骨的动力学
- 分子细胞生物学分子细胞生物学
背景情况:
- 体是富含动氨酸的结构,对细胞粘附,迁移和细胞外基质降解至关重要.
- 连接受体细胞核与等离子体膜的精确机制尚不完全理解.
研究的目的:
- 调查I类肌蛋白 (Myo1e和Myo1f) 在受体结构和功能中的作用.
- 为了确定Myo1e和Myo1f定位的特定的Podosome区域,以及它们对膜附着的贡献.
主要方法:
- 在巨细胞中利用了Myo1e和Myo1f的淘汰和淘汰策略.
- 通过先进的显微镜技术分析了体形态学,行为动力学和细胞迁移.
- 研究了Myo1e/f在子体内的定位,专注于它们的TH2域.
主要成果:
- Myo1e 和 Myo1f 定位在受体体的近膜区域,称为"基".
- 丧失Myo1e/f功能会导致较大的受体体与改变的actin周转率和横向移动性.
- Myo1e/f 缺乏会损害 3D 和 2D 细胞迁移,但会增强细胞外基质的降解.
结论:
- 一类的myosinsMyo1e和Myo1f是podosome附着到等离子体膜的关键调节者.
- 这些肌酸蛋白定义了一个新的受体亚体结构,即"基础",对于适当的受体功能和细胞迁移至关重要.
- Myo1e/f在平衡细胞迁移和矩阵降解活动方面发挥着至关重要的作用.
相关概念视频
01:18Role of Myosin in Cell Migration
3.2K
Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
3.2K
01:35Cancer Cell Migration through Invadopodia
3.2K
Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.2K
01:10The Role of Actin and Myosin in Non-muscle Cells
4.6K
Actin and myosin or actomyosin filaments also play a significant role in cells other than those involved in muscle contraction (which occurs within the sarcomere of muscle cells). The mechanism of non-muscle cell contractile bundles was first observed in Dictyostelium and Acanthamoeba. In non-muscle cells, two bundles are commonly found: stress fibers and actomyosin adherence belts. These contractile bundles are smaller and less organized than the ones found in muscle cells. They are held...
4.6K
01:39Mechanism of Filopodia Formation
3.1K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
3.1K
01:31Mechanism of Lamellipodia Formation
3.6K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
3.6K
01:13Actin Polymerization and Cell Motility
6.5K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
6.5K