过氧化素6 (Prdx6) 和死后牛肉软化中的氧化应激:一种蛋白质学视角
Huixin Zuo1, Linlin Huang2, Mingming Huang1
1College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.
Journal of proteomics
|September 11, 2025
概括
用mercaptosuccinic acid (MA) 抑制氧化素6 (Prdx6) 活性,通过增加氧化应激和蛋白质分解,加速牛肉软化. 这提供了一种减少肉类老化时间和成本的策略.
科学领域:
- 肉类科学和死后老化研究.
- 氧化应激和蛋白质降解的生物化学.
- 肉类软化的分子机制.
背景情况:
- 肉类软化是一种复杂的死后老化过程,受酶和非酶因素的影响.
- 氧化6 (Prdx6) 呈现非谷过氧化酶 (NSGPx) 活性,可能影响肉质.
- 了解Prdx6的作用对于开发优化牛肉老化和嫩度的策略至关重要.
研究的目的:
- 调查Prdx6介导的NSGPx活性在牛肉在死后老化过程中的软化中的作用.
- 评估氧化应激调节剂 (H2O2,NAC,MA) 对Prdx6活性和肉质的影响.
- 识别与加速软化相关的分子标记物.
主要方法:
- 在牛肉中分析剪切力,NSGPx活性和蛋白质丰度 (HSP70,HSP27,Troponin-T).
- 用过氧化 (H2O2),N-乙半氨酸 (NAC),酸 (MA) 或盐水 (控制) 治疗牛肉肌肉.
- 基于Tandem Mass Tag (TMT) 的蛋白质组学,以识别差异丰富的蛋白质.
主要成果:
- 药物治疗抑制了NSGPx的活性,加速了软化,提高了HSP70的调节,并增加了托罗-T的分解.
- H2O2上调 HSP70 和 HSP27 在死后6-12小时内.
- 蛋白质组学确定了与细胞骨,细胞外基质,氨基酸代谢和亡途径相关的蛋白质.
结论:
- Prdx6活性显著调节影响牛肉软化的氧化应激路径.
- 用MA抑制Prdx6会增强氧化应激,加速托罗-T蛋白解和肉的柔软性.
- 治疗MA提供了一种可行的策略,可以在72h post-mortem内将牛肉的老化时间减少20%以上.
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