Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Distribution Reliability and Automation01:25

Distribution Reliability and Automation

498
Distribution reliability in electrical power systems is critical for ensuring an uninterrupted power supply to consumers at minimal cost. According to IEEE Standard Terms, reliability is the probability that a device will function without failure over a specified time period or amount of usage. For electric power distribution, this translates to maintaining continuous power supply and addressing customer concerns over power outages. Several indices, as defined by IEEE Standard 1366-2012, are...
498
Production of Formed Elements01:34

Production of Formed Elements

3.6K
Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...
3.6K
Multimachine Stability01:25

Multimachine Stability

548
Multimachine stability analysis is crucial for understanding the dynamics and stability of power systems with multiple synchronous machines. The objective is to solve the swing equations for a network of M machines connected to an N-bus power system.
In analyzing the system, the nodal equations represent the relationship between bus voltages, machine voltages, and machine currents. The nodal equation is given by:
548
Production Efficiency01:01

Production Efficiency

18.2K
Net production efficiency (NPE) is the efficiency at which organisms assimilate energy into biomass for the next trophic level. Due to low metabolic rates and less energy spent on thermoregulatory processes, the NPE of ectotherms (cold-blooded animals) is 10 times higher than endotherms (warm-blooded animals).
18.2K
PD Controller: Design01:26

PD Controller: Design

627
In automotive engineering, car suspension systems often employ Proportional Derivative (PD) controllers to enhance performance. PD controllers are utilized to adjust the damping force in response to road conditions. A controller, acting as an amplifier with a constant gain, demonstrates proportional control, with output directly mirroring input.
Designing a continuous-data controller requires selecting and linking components like adders and integrators, which are fundamental in Proportional,...
627
Distributed Loads01:19

Distributed Loads

950
Distributed loads are a common type of load that engineers and scientists encounter in various practical situations. Distributed loads often refer to a type of load spread over a surface or a structure and can be modeled as continuous force per unit area.
For example, consider a bookshelf filled with books stacked vertically adjacent to each other. The weight of the books is evenly distributed over the length of the shelf. As a result, the pressure at different locations on the surface of the...
950

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Clinical Supervision and Positive Practice Environments: A Scoping Review.

Portuguese journal of public health·2026
Same author

High-Yield Production and Cost-Effective Purification of an Alkali-Resistant Hexameric Protein A Variant for Antibody Affinity Chromatography.

Biotechnology journal·2026
Same author

Real-world outcomes and prognostic factors in anaplastic thyroid cancer: evidence from the REGETNE-Thyroid cohort.

The oncologist·2026
Same author

One-Pot Synthesis of a Small Synthetic Ligand for Antibody Purification.

Biotechnology and bioengineering·2026
Same author

Ferritin nanoparticles displaying rift valley fever virus glycoprotein elicit potent dendritic cell activation in vitro.

Virology journal·2026
Same author

Bioengineered baculovirus-derived extracellular vesicles loaded with of γ-carboxylated Gla-rich protein: Dual modulation of inflammation and vascular calcification.

Biomaterials advances·2026
Same journal

High-level De novo Biosynthesis of Plant-Derived Pinosylvin in Yarrowia lipolytica via Metabolic Engineering and Surfactant-Mediated Fermentation.

Journal of biotechnology·2026
Same journal

The effect of reduced graphene oxide in chitosan-based nanoparticles on the enzymatic properties of the immobilized enzyme.

Journal of biotechnology·2026
Same journal

High-level biosynthesis of gastrodin in engineered Escherichia coli.

Journal of biotechnology·2026
Same journal

From plasmid sequence to process design: A computational analysis of metabolism in the context of plasmid DNA manufacturing.

Journal of biotechnology·2026
Same journal

Development of an inducible cellobiohydrolase promoter and its application for enhancing the production of ganoderic acids in Ganoderma lingzhi.

Journal of biotechnology·2026
Same journal

Malic acid and allied exogenous chemicals induces desaturase gene expression and elevates PUFA production in marine microalgae Isochrysis sp.

Journal of biotechnology·2026
查看所有相关文章

相关实验视频

Updated: Jan 18, 2026

Author Spotlight: Advancing Gene Therapy Research with High-Titer Adeno-Associated Virus Vector Production
05:51

Author Spotlight: Advancing Gene Therapy Research with High-Titer Adeno-Associated Virus Vector Production

Published on: May 3, 2024

2.6K

为AAV8生产开发一个强大且可扩展的平台.

André Nascimento1, Tiago Q Faria1, Tiago Nunes1

  • 1iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, Oeiras 2780-901, Portugal; ITQB-NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, Oeiras 2780-157, Portugal.

Journal of biotechnology
|September 11, 2025
PubMed
概括
此摘要是机器生成的。

这项研究开发了一种可扩展的腺相关病毒 (AAV) 基因治疗生产平台,从2L到50L尺度实现一致的高产量和产品质量. 过程优化提高了经济效益,而不会影响病毒载体生产效率.

关键词:
腺相关病毒的病毒.生物工艺的稳固性 生物工艺的稳固性通过全囊酸丰富进行了丰富.净化 净化 净化 净化可扩展性 可扩展性

更多相关视频

Process Development for the Production and Purification of Adeno-Associated Virus AAV2 Vector using Baculovirus-Insect Cell Culture System
10:31

Process Development for the Production and Purification of Adeno-Associated Virus AAV2 Vector using Baculovirus-Insect Cell Culture System

Published on: January 13, 2022

10.3K
Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models
07:21

Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models

Published on: June 30, 2021

7.1K

相关实验视频

Last Updated: Jan 18, 2026

Author Spotlight: Advancing Gene Therapy Research with High-Titer Adeno-Associated Virus Vector Production
05:51

Author Spotlight: Advancing Gene Therapy Research with High-Titer Adeno-Associated Virus Vector Production

Published on: May 3, 2024

2.6K
Process Development for the Production and Purification of Adeno-Associated Virus AAV2 Vector using Baculovirus-Insect Cell Culture System
10:31

Process Development for the Production and Purification of Adeno-Associated Virus AAV2 Vector using Baculovirus-Insect Cell Culture System

Published on: January 13, 2022

10.3K
Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models
07:21

Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models

Published on: June 30, 2021

7.1K

科学领域:

  • 生物技术是生物技术.
  • 基因治疗制造业 基因治疗制造业
  • 病毒载体生产病毒载体生产

背景情况:

  • 腺相关病毒 (AAV) 载体对于基因疗法至关重要,需要稳健可扩展的制造工艺.
  • 目前对AAV载体的需求需要高效和可重复的生产平台.

研究的目的:

  • 设计和实施一个可扩展的AAV8生产平台.
  • 验证工艺的稳定性和可扩展性,从实验室到试点规模.
  • 在保持产品质量的同时,优化工艺经济性.

主要方法:

  • 在2升和50升尺度上进行了三次生产活动.
  • 评估上游和下游的过程参数,以获得一致性和产量.
  • 评估了关键的修改,包括注射度,核酶选择和直接色谱加载.

主要成果:

  • 在各个尺度中实现了一致的标题和恢复率接近80%.
  • 验证了成功的可扩展性从2L到50L,而不会损失性能.
  • 证明了全片的三倍丰富,表明产品质量更高.
  • 在不影响生产的情况下确定了节省成本的修改.

结论:

  • 开发的AAV8生产平台是强大的,可扩展的,经济可行的.
  • 该过程确保了高产量和产品质量,并有可能在AAV血清型中广泛应用.
  • 优化生物处理有助于基因治疗制造业的进步.