可持续地保护多种宿主免受多虫害害,使用基于植物益生菌的基因沉默
Jing Chen1, Rong Zhang1, Xun Zhang2
1Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, 100091, China.
Trends in biotechnology
|September 11, 2025
概括
这项研究设计了一个Bacillus益生菌来表达dRNA用于害虫控制. 修改后的细菌有效地沉默了害虫基因,提供了可持续的,广泛的植物保护,防止秋季网虫.
科学领域:
- 农业昆虫学 农业昆虫学
- 分子生物学分子生物学
- 微生物生物技术 微生物生物技术
背景情况:
- RNA干扰 (RNAi) 是一种抑制基因的机制,具有害虫控制的潜力.
- 目前的RNAi策略面临着持久性和频谱的限制,阻碍了对多虫害的有效性.
- 开发可持续和广泛的害虫防治解决方案对农业至关重要.
研究的目的:
- 开发一种新的RNAi传递系统,使用植物益生菌进行可持续的害虫控制.
- 为了设计一种Bacillus菌株,以表达针对多虫害虫Hyphantria cunea的dsRNA.
- 评估植物保护工程益生菌的疗效和现场性能.
主要方法:
- 隔离和基因改造一种Bacillus菌株以表达针对H. cunea.的dsRNA.
- 评估工程菌株对害虫化率和死亡率的影响.
- 评估工程菌株在野外条件下在宿主植物中的殖民和持久性.
- 对dsrna对害虫能量代谢和皮层形成的影响的分析.
主要成果:
- 改造的Bacillus菌株 (SH-F8) 显著降低了H. cunea的繁殖和增加了死亡率.
- 工程dsRNA破坏了关键的害虫过程,包括能量代谢和皮层形成.
- 在现场条件下证实了SH-F8在两个宿主植物物种的成功殖民.
- 在高温/高湿度条件下观察到增强的益生菌种群密度.
结论:
- 基于植物益生菌的基因沉默 (PPGS) 提供了一种可持续的方法来保护多种植物免受多虫害害.
- 工程 Bacillus 益生菌可以有效地传递 dsRNA,以对害虫进行向基因沉默.
- 这一战略对开发环保和持久性害虫管理解决方案具有前景.
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