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可光切换的Intin用于控制共价蛋白结合和裂变的光控制

Mikhail Baloban¹, Kyrylo Yu Manoilov¹, Maksim M Karasev²

¹Department of Genetics, and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, USA.

Nature communications

|September 11, 2025

概括

研究人员开发了一种可光切换的蛋白质 (PS Intein),用于精确的光控制蛋白质操纵哺乳动物细胞. 这种新的光遗传工具提供了卓越的功能和低背景,使先进的细胞过程调节.

科学领域:

生物化学生物化学
分子生物学分子生物学
视觉遗传学视觉遗传学

背景情况:

在哺乳动物细胞中进行蛋白质操纵的光遗传工具面临着稳定性差和效率低等挑战.
现有的方法通常需要非天然的氨基酸,限制了它们的适用性.

研究的目的:

设计一种新型的可光切换的整蛋白 (PS Intein),用于精确控制哺乳动物细胞中的蛋白质结合和裂变.
克服当前光遗传工具的局限性,提供增强的功能和减少的背景活动.

主要方法:

通过对gp41-1蛋白质与Vivid光受体进行合调节,设计了一种可光开关的整蛋白 (PS Intein).
测试了PS Intein在哺乳动物细胞中的功能,稳定性和背景活性.
展示了基于PS 蛋白质的系统,用于光诱导的蛋白质结合,分裂和释放,以调节基因表达和细胞命运.

主要成果:

与现有的光遗传工具相比,PS Intein表现出卓越的功能和显著较低的背景活动.
基于PS 蛋白质的系统可实现高效的光诱导共价结合,分裂和蛋白质的释放.
该技术促进了光控制的基因表达和细胞命运的调节,并有可能对交叉细胞进行向.

结论:

PS Intein代表了一种多功能和高效的技术,用于在活细胞中以光控制对蛋白质进行操纵.
这种工具可以精确控制蛋白质网络,从而在细胞生物学和生物技术中实现新的功能和应用.
PS Intein系统克服了当前光遗传学方法的关键局限性,为先进的细胞工程铺平了道路.

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