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乳酸细菌的功能性质从塔拉纳样本中分离出来的后生物

Ecem Aydin¹, Çisem Bulut Albayrak², Olcay Boyacioğlu³

¹Graduate School of Natural and Applied Sciences, Aydın Adnan Menderes University, Food Engineering Program, 09010, Aydın, Efeler, Turkey.

Probiotics and antimicrobial proteins

|September 11, 2025

概括

来自土耳其tarhana的后生物质,作为无细胞超级生物或热杀死细胞产生,显示对有害细菌的抗菌活性. 这些化合物,主要是酸性,提供潜在的食品和健康行业应用.

科学领域:

食品科学与技术食品科学与技术
微生物学微生物学
生物技术是生物技术.

背景情况:

土耳其传统的发酵Tarhana是乳酸细菌 (LAB) 的来源.
后生物质,即从微生物中获得的有益化合物,正作为益生菌的替代品获得越来越多的兴趣.

研究的目的:

从tarhana中分离的LAB衍生的后生物的特征.
评估这些后生物质的抗微生物和抗氧化特性.
评估它们的安全性,并比较不同的生产方法 (无细胞超级生物与热杀死细胞).

主要方法:

乳酸细菌从tarhana.com中分离出来.
后生物质被制备为无细胞超级生物 (CFS) 和热杀死 (HK) 细胞碎片.
使用井扩散方法对食源性病原体进行了抗菌活性测试.
使用DPPH激素清除试验评估了抗氧化活性.
在HT-29细胞上评估了细胞毒性.
化学分析包括HPLC和FT-IR.

主要成果:

后生物体对黄金葡萄球菌和Listeria monocytogenes表现出显著的抗菌活性.
抗菌活性归因于酸性化合物.
已识别的Pediococcus pentosaceus菌株产生了最强大的后生物.
后生物药物表现出抗氧化活性,并以剂量依赖的方式降低了癌细胞活力.
在CFS和HK后生物形式之间没有发现活性或结构的显著差异.

结论:

来自tarhana LAB的后生物质具有显著的抗微生物和抗氧化特性.
两种形式的CFS和HK都是有效的,这表明在应用中具有灵活性.
这些发现突出了tarhana衍生后生物的食品和健康行业的潜力.

关键词:

这是一种抗癌药物.抗微生物药物抗微生物药物抗氧化剂是一种抗氧化剂.没有细胞的超生体.被热杀死的人.实验室实验室实验室实验室

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