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血液和组织的下一代测序之间的诊断性能的比较,用于原发性脊髓感染

Chang Liu^1,2, Jiang Long^1,2, Yuan Li^1,2

¹Department of Orthopedics Xinqiao Hospital, Army Medical University, Chongqing, People's Republic of China.

Global spine journal

|September 12, 2025

概括

与组织mNGS相比,血液元基因组下一代测序 (mNGS) 对原发性脊髓感染的诊断价值有限. 组织mNGS为这些严重感染提供了卓越的病原体检测.

科学领域:

传染性疾病传染性疾病
微生物学微生物学
基因组学就是基因组学.

背景情况:

从遥远的部位出血的传播是脊髓感染的主要原因.
甲基因组下一代测序 (mNGS) 是一种有前途的病原体检测工具.
血液mNGS用于诊断原发性脊髓感染的实用性需要研究.

研究的目的:

为了比较血液mNGS与组织mNGS在原发性脊髓感染中的诊断性能.
为了评估血液mNGS与传统的血液和组织病原体培养.

主要方法:

一项前性研究分析了21名患有原发性脊髓感染的患者.
血液和组织mNGS和病原体培养结果的比较.
诊断效率参数的计算,包括阳性率,灵敏度和特异性.

主要成果:

组织mNGS表现出明显更高的阳性率,灵敏度和特异性比血液mNGS.
血液mNGS显示的阳性率和灵敏度高于血液病原体培养,但灵敏度和特异性低于组织培养.
血液mNGS在四种测试的病原体识别技术中表现出最低的特异性.

结论:

血液mNGS在诊断原发性脊髓感染方面表现不如组织mNGS.
在原发性脊髓感染中血液mNGS的诊断应用目前有限.
需要进一步的研究,以探索其他脊髓感染类型或特定患者亚组的血液mNGS.

关键词:

血液血液血液血液诊断诊断诊断诊断诊断诊断这是下一代测序.一次性脊髓感染.

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