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相关概念视频

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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相关实验视频

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Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
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模块化,开源的多重复合用于民主化空间信息.

Nicholas Zhang1,2,3, Zhou Fang1,3, Priyam Kadakia1,3

  • 1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA. ahmet.coskun@bme.gatech.edu.

Lab on a chip
|September 12, 2025
PubMed
概括
此摘要是机器生成的。

研究人员开发了PRISMS,这是一个开源的,自动化的空间omics管道. 这种具有成本效益的解决方案使生物发现的多重成像利用机器人和简化显微镜实现了民主化.

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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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科学领域:

  • 空间奥米克斯 空间奥米克斯
  • 分子成像学分子成像学
  • 生物技术是生物技术.

背景情况:

  • 空间奥米克能够在本地组织环境中可视化生物分子.
  • 专有工具的高成本限制了空间空间技术的可访问性.
  • 需要为多重成像提供具有成本效益和可重复性的解决方案.

研究的目的:

  • 介绍PRISMS (基于Python的机器人成像和模块化空间omics的染色),一个开源的自动化复杂化管道.
  • 通过提供可访问,可复制和具有成本效益的解决方案来民主化空间空间.
  • 能够实现高通量单分子光成像和超高分辨率成像.

主要方法:

  • 使用具有热控制的液体处理机器人进行自动RNA和蛋白质染色.
  • 开发了模块化样本持有器和Python控制器,用于高通量成像.
  • 集成与宽场和共聚焦显微镜以及尼康NIS Elements软件.

主要成果:

  • 通过成像组织幻灯片和附着细胞来证明PRISMS的多功能性.
  • 成功执行超分辨率成像,包括超分辨率辐射波动 (SRRF).
  • 实现了高通量,单分子光成像.

结论:

  • PRISMS通过一个可访问,可复制和具有成本效益的平台,使空间信息民主化.
  • 普里斯姆的开源性质促进了空间生物学领域的更广泛的采用和创新.
  • PRISMS使研究人员能够推进空间生物学,并进行新的发现.