电子烟蒸汽在对LPS的反应中放大了中性恋炎症和蛋白质溶解EVs
Lawrence W Rasmussen1,2, Dakota C Finley1, Julian B Smith1
1Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL, United States.
电子烟蒸汽为肺部准备严重的炎症和损伤,当暴露于随后的挑战,如感染. 电子烟可能通过放大对其他压力因素的炎症反应来加剧肺部疾病.
科学领域:
- 肺部医学 肺部医学
- 免疫学 免疫学 免疫学
- 毒理学 毒理学 毒理学
背景情况:
- 电子烟的使用 (vaping) 已经增加,但其对呼吸道炎症和细胞外囊泡 (EV) 生物学的影响尚不清楚.
- 这项研究调查了电子烟对肺部免疫反应,中性恋炎症和EV蛋白质分解活性的影响.
研究的目的:
- 为了确定长期和急性电子烟蒸汽暴露如何影响肺部免疫力.
- 评估细胞外囊泡在蒸汽诱导的肺损伤中的作用.
- 评估在蒸汽暴露和随后的挑战后,中性粒细胞衍生的EVs的致病潜力.
主要方法:
- 小鼠每天暴露在蒸汽中长达12周,随后对免疫细胞和EVs进行评估.
- 在两周的蒸汽暴露后,对模型的二次肺损伤进行了脂聚糖化物 (LPS) 挑战.
- 从中性粒细胞衍生的EVs (nEVs) 被分离并转移到原始小鼠中,以评估它们的致病潜力.
主要成果:
- 单独暴露于蒸汽并没有显著改变免疫细胞透,肺组织学或EV蛋白酶活性.
- 用LPS挑战的蒸汽预暴露小鼠表现出增加的中性粒细胞透,在EV中中性粒细胞弹性酶活性升高,以及更大的膜损伤.
- 从暴露于蒸汽的,受到LPS挑战的小鼠中转移的NEVs在原始小鼠中诱导了更严重的肺变化.
结论:
- 电子烟蒸汽启动了免疫系统,放大了对随后挑战的炎症和破坏性反应.
- 当与感染或其他环境压力因素相结合时,电子烟可能会加剧肺部损伤.
- 这些发现引发了人们对电子烟对肺部疾病恶化的贡献的担忧.
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