在RuvA寡合化和RuvAB介导的分支迁移中,阿尔金宁-121的功能影响在格兰阳性Listeria monocytogenes中
Deeksha Sugunan1,2, Piero R Bianco3, K Neelakanteshwar Patil1,2
1Department of Microbiology and Fermentation Technology, Council of Scientific and Industrial Research-Central Food Technological Research Institute (CSIR-CFTRI), Mysuru, 570020, Karnataka, India.
FEBS letters
|September 12, 2025
概括
在Listeria monocytogenes中RuvA蛋白质作为一个八度体,而不是四度体,用于DNA修复. 影响八合体形成的突变阻止了必要的分支迁移,为抗生素耐药性策略提供了潜在的目标.
科学领域:
- 分子生物学分子生物学
- 微生物学 微生物学
- DNA 修复机制的修复机制
背景情况:
- 鲁瓦B复合物促进了 prokaryotes 中的霍莱德结 (HJ) 分支迁移.
- 在阳性细菌中,RuvA的寡合状态 (四聚体与八聚体) 尚不清楚.
- 李斯特菌 (Listeria monocytogenes) 是一种重要的食物传播病原体,对抗生素耐药性有影响.
研究的目的:
- 在分支迁移中研究来自Listeria monocytogenes (LmRuvA) 的RuvA的功能寡合状态.
- 为了确定参与LmRuvA寡合化和功能的关键残留物.
主要方法:
- 针对LmRuvA的局部导向突变发生,重点是阿尔金宁-121.
- 在溶液中使用生物化学分析分析LmRuvA寡合物状态.
- 评估野生类型和突变LmRuvA的HJ结合和分支迁移活动.
- 评估LmRuvA与RuvB蛋白的相互作用.
主要成果:
- -121对于四聚体-四聚体接口形成至关重要,对于八聚体组装至关重要.
- 一种突变的LmRuvA (R121D) 呈现二聚四聚平衡,仅作为四聚分子结合HJs,并显示与RuvB.的相互作用减少.
- R121D突变LmRuvA在催化分支迁移方面存在缺陷.
结论:
- LmRuvA需要一个八度的形式,以有效地结合霍莱德交叉点,并驱动分支迁移.
- 在Listeria monocytogenes中,LmRuvA的八度状态对其DNA修复功能至关重要.
- 准LmRuvA寡合化是一种潜在的策略,可以对抗这种病原体的抗生素耐药性.
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