PARP14是一种干扰素诱导的宿主因子,促进IFN的产生,并影响多种病毒的复制
Srivatsan Parthasarathy1, Pradtahna Saenjamsai1, Hongping Hao1
1Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, USA.
mBio
|September 12, 2025
概括
聚 ((ADP-ribose) 聚合酶14 (PARP14) 蛋白增强干扰素的产生,并限制冠状病毒和简单疹病毒1的复制. 然而,PARP14还促进囊泡性口炎病毒的复制,揭示了宿主-病原体相互作用中的双重作用.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 生物化学 生物化学
背景情况:
- 聚(ADP-ribose) 聚合酶14 (PARP14) 是一种ADP-ribosyltransferase,参与DNA损伤,炎症和癌症.
- PARP14是由干扰素 (IFN) 上调调节的,并显示出积极选择的迹象,这表明它在宿主-病原体冲突中发挥了作用.
- 以前的研究表明,PARP14在冠状病毒感染期间参与IFN-I的产生,但其直接的抗病毒功能仍不清楚.
研究的目的:
- 研究PARP14对各种病毒的直接抗病毒功能.
- 为了确定PARP14的催化活性是否对其抗病毒作用至关重要.
- 阐明PARP14在病毒感染中的双重前病毒和抗病毒作用.
主要方法:
- 使用PARP14淘汰赛 (KO) A549细胞来评估病毒复制.
- 感染了不同病毒的KO细胞:ARH缺乏的MHV,SARS-CoV-2,HSV-1,VSV和LCMV.
- 采用PARP14活性部位抑制剂来评估催化活性的作用.
主要成果:
- PARP14的催化活性增强了IFN-β和IFN-γ的反应.
- PARP14限制了ARH缺乏MHV,SARS-CoV-2和HSV-1的复制.
- PARP14增强了VSV的复制,而LCMV的复制不受影响.
- PARP14对HSV-1和VSV的影响独立于其催化活性.
结论:
- PARP14既具有抗病毒功能 (限制CoV和HSV-1),也具有前病毒功能 (增强VSV复制).
- PARP14在调节病毒感染方面发挥着复杂的作用,影响多种病毒类型.
- 进一步研究PARP14的机制对于理解它在宿主-病原体相互作用中的作用和开发抗病毒策略至关重要.
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