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Updated: Jan 18, 2026

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
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基于CRISPR/Cas13系统的驱动DNA酶开关驱动DNA步行系统,用于敏感和直接检测轮状病毒

Xiaozheng Kang1, Fanglu Tian2, Xiaogang Liu3

  • 1Emergency Department, School of Medicine, Sichuan Academy of Medical Sciences, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu City, Sichuan Province, 610000, China.

Mikrochimica acta
|September 12, 2025
PubMed
概括

这项研究引入了一种创新的DNA探针,该探针将驱动放大 (EDA) 与DNAzyme驱动的DNA步行器相结合,用于敏感的轮状病毒检测. 该系统实现了高特异性和灵敏性,检测极限为2.7 fM.

关键词:
在Cas13a/crRNA中使用.一个DNA步行者.这就是DNAzyme DNA酶.由值驱动的放大效应是以值驱动的.罗塔病毒 (Rotavirus) 是一种病毒.

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