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使用微流体装置并行测量多个芽酵母菌株对细胞外变化的反应的协议
Ivan B N Clark1, Matthew M Crane1, Julian M J Pietsch1
1Centre for Engineering Biology, University of Edinburgh, Edinburgh EH9 3BF, Scotland, UK.
STAR protocols
|September 12, 2025
概括
这项研究引入了一种多腔室微流体装置,用于长期对芽酵母的单细胞分析. 该协议可以同时监测多个酵母菌株的生长和反应.
科学领域:
- 微流体学 微流体学
- 细胞生物学 细胞生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 长期的单细胞测量对于理解细胞动态至关重要.
- 像Alcatras这样的现有微流体装置通过固定细胞来促进单细胞研究.
- 在酵母研究中,同时监测多个菌株是一个挑战.
研究的目的:
- 介绍一个多腔室微流体装置的协议.
- 为了能够同时监测多达五种芽酵母菌株的生长和反应.
- 为设备准备,细胞加载和操作提供详细的步骤.
主要方法:
- 使用了阿尔卡特拉斯微流体装置的多室版本.
- 开发了一个准备对数酵母培养和中等水库的协议.
- 设备填充,电池加载,管道设置和介质流动启动的详细程序.
主要成果:
- 成功调整了阿尔卡特拉斯装置用于多菌株,单细胞分析.
- 建立了一个全面的协议,用于同时监测芽酵母.
- 能够长期观察多个菌株的细胞生长和反应.
结论:
- 本协议提供了一种可靠的方法,用于在芽酵母中进行高通量单细胞分析.
- 这种微流体方法促进了不同酵母菌株的比较研究.
- 该方法对于推进酵母细胞生物学和遗传学的研究至关重要.
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