Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Engineering programmable tumor microenvironment interactions through single-cell bioprinting of spatially defined cell microarrays.

Biofabrication·2026
Same author

Engineered muscle drives bone repair and functional healing after composite musculoskeletal injury.

Biomaterials·2026
Same author

Engineering bioinspired, high-density collagen microgels with tunable intrafibrillar mineralization for accelerated osteogenesis in vitro and bone regeneration in vivo.

Acta biomaterialia·2026
Same author

Endogenous Osteocyte-Osteoclast Signaling Enables Growth Factor-Free Bone Remodeling, Drug Response, and Cancer Invasion in a Nanoscale Calcified Bone-on-a-Chip Model.

bioRxiv : the preprint server for biology·2025
Same author

Strategies for Craniofacial Tissue Engineering: Innovations for Scalable Bone Regeneration.

Plastic and aesthetic research·2025
Same author

Host-microbe-cancer interactions on-a-chip.

Frontiers in bioengineering and biotechnology·2025

相关实验视频

Updated: Jan 18, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

7.1K

关于培养细胞和人工组织的单细胞空间转录形状分析的协议,不进行嵌入或切割.

Haylie R Helms1, Luiz E Bertassoni2

  • 1Department of Biomedical Engineering, School of Medicine, Oregon Health and Science University, Portland, OR 97201, USA; Knight Cancer Precision Biofabrication Hub, Knight Cancer Institute, Oregon Health and Science University, Portland, OR 97201, USA; Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health and Science University, Portland, OR 97201, USA.

STAR protocols
|September 12, 2025
PubMed
概括

我们开发了一种新的协议,用于对二维工程组织和细胞培养物的空间转录组分析. 这种方法与Visium HD空间技术兼容,可实现详细的细胞洞察.

关键词:
生物技术和生物工程癌症 癌症 癌症 癌症细胞生物学 细胞生物学细胞培养培养的细胞培养.基于细胞的测试基因表达 基因表达基因组学就是基因组学.显微镜的使用方法序列分析是指进行序列分析.序列化是指测序的使用.一个单细胞的单细胞.组织工程是组织工程.

更多相关视频

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

30.6K
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.4K

相关实验视频

Last Updated: Jan 18, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

7.1K
Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

30.6K
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.4K

科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 基因组学就是基因组学.

背景情况:

  • 空间转录学揭示了细胞组成和影响细胞行为的空间关系.
  • 标准协议通常需要嵌入和切割,限制对2D工程组织和细胞培养物的分析.

研究的目的:

  • 介绍一项用于二维工程组织和细胞培养物的空间转录基因分析的新方案.
  • 调整现有的方法,使其与Visium HD空间技术兼容.

主要方法:

  • 提供了样品生成,固定和染色的详细步骤.
  • 该协议是为与标准嵌入和切割不兼容的2D工程组织和细胞培养设计的.
  • 与Visium HD空间技术的集成是一个关键功能.

主要成果:

  • 一个灵活和有效的协议,用于对具有挑战性的2D样本进行空间转录组分析.
  • 空间转录学成功适应工程组织和细胞培养.
  • 能够对细胞微环境进行详细的研究.

结论:

  • 该协议将空间转录学的应用扩展到更广泛的生物系统.
  • 它为研究人员在2D培养和工程组织中研究细胞相互作用提供了有价值的工具.
  • 促进对空间基因表达模式的更深入的理解.