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相关概念视频

Reporter Genes02:11

Reporter Genes

12.9K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.3K
DNA Microarrays02:34

DNA Microarrays

20.7K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
20.7K

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相关实验视频

Updated: Jan 18, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
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Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

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用化学遗传记者系统对基因表达进行双色时间标记.

Henriette Lämmermann1, Jade Nguyen2, Juan F Tamez-Fernández1

  • 1Department of Chemistry, University of Zurich, 8057, Zurich, Switzerland.

Chembiochem : a European journal of chemical biology
|September 12, 2025
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种新的记者测试来监测BiP/GRP78表达,这是展开蛋白质反应的关键部分. 该工具发现了一种新的BiP/GRP78激活剂,有助于应激反应研究.

关键词:
BiP/GRP78的使用情况这是HaloTag的标签.高含量查的高含量查记者基因测试 记者基因测试

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Using Coculture to Detect Chemically Mediated Interspecies Interactions
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Using Coculture to Detect Chemically Mediated Interspecies Interactions

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Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
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Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

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相关实验视频

Last Updated: Jan 18, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
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Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

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Using Coculture to Detect Chemically Mediated Interspecies Interactions
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Using Coculture to Detect Chemically Mediated Interspecies Interactions

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Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
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Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

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科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 应激反应途径对于细胞平衡至关重要.
  • 细胞内膜网中的未折叠蛋白质反应 (UPR) 维持了蛋白质的折叠和加工.
  • BiP/GRP78是UPR的关键调节者,监测其表达至关重要.

研究的目的:

  • 开发一种用于监测BiP/GRP78表达的新型化学遗传记者试验.
  • 为了能够灵活和灵敏地检测活细胞中的BiP/GRP78水平.
  • 通过高含量查识别BiP/GRP78表达的新型激活剂.

主要方法:

  • 开发一个使用HaloTag通过内部核糖体入口部位共同表达的记者系统.
  • 实施双色计时策略,以提高读出灵敏度.
  • 记者系统在活细胞成像,流细胞计和高含量查中的应用.

主要成果:

  • 成功建立了BiP/GRP78表达的功能化遗传记者试验.
  • 该测试显示了标签的灵活性和通过双色时间印策略提高了灵敏度.
  • 使用报告员系统的高内容屏幕发现了BiP/GRP78表达的新且有前途的激活剂.

结论:

  • 开发的报告员系统为研究展开的蛋白质反应提供了一个多功能和灵敏的工具.
  • 这种测试有助于发现细胞应激通路的新调节剂.
  • 已识别的BiP/GRP78激活剂在与蛋白质折叠相关的疾病中具有治疗应用的潜力.