半氨酸反应性分析揭示了单克隆抗体二硫化物结合降低机制在生物制药过程中中间体

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Updated: Jan 18, 2026

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
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Published on: May 2, 2019

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Taku Tsukidate1, Zhenshu Wang2, Andrew Hsieh2

  • 1Analytical Research & Development Mass Spectrometry, Merck & Co., Inc., 126 East Lincoln Avenue, Rahway, New Jersey 07065, United States.

Journal of the American Society for Mass Spectrometry
|September 12, 2025
PubMed
概括

这项研究开发了一种化学蛋白质学工作流程,以了解在中国仓鼠卵巢 (CHO) 细胞生产过程中,单克隆抗体 (mAbs) 中二硫化键如何减少. 这些发现揭示了参与这种减少过程的关键酶,有助于生物治疗制造.

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