麦中的化化合物的发芽驱动的动态变化以及抗氧化增强的潜在机制
Manting Huang1, Qiaochun Chen1, Siqian Li2
1Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China.
Food chemistry
|September 12, 2025
概括
发芽麦显著增加了多醇含量和抗氧化活性. 不同的发芽阶段会产生不同的基配置,通过功能性食物发育的特定信号通路影响细胞的抗氧化反应.
科学领域:
- 营养生物化学和食品科学
- 植物生理学和分子生物学.
背景情况:
- 麦发芽是一种有前途的策略,可以提高营养价值,特别是化合物.
- 对于功能性食品应用来说,了解麦基因和其生物活性的时间依赖性变化至关重要.
研究的目的:
- 为了研究在发芽期间麦烯化合物的时间依赖性变化.
- 将这些转移与抗氧化能力和潜在的细胞机制联系起来.
- 为了确定特定的功能性食品应用的最佳发芽终点.
主要方法:
- 在9天的发芽过程中,总聚和特定的化合物 (青胺,类C-糖化物) 的量化.
- 使用DPPH和ABTS激素清除方法对抗氧化能力的评估.
- 在RAW264.7巨细胞中评估细胞抗氧化作用,包括ROS水平,抗氧化酶活性 (SOD,GPx,CAT) 和信号通路激活 (Nrf2,HO-1,NQO-1,AMPK,PI3K).
主要成果:
- 发芽9天后,多的总含量增加了四倍.
- 青胺在早期发芽 (3天) 中占主导地位,而黄类C-糖化物在后期阶段 (7-9天) 成为主要的.
- 发芽的麦表现出显著更高的抗氧化能力和减少细胞氧化应激,基于基配置文件的独特信号通路激活.
结论:
- 发芽显著重塑麦的烯基成分,增强抗氧化特性.
- 早期发芽 (富含青胺) 激活AMPK/HO-1,而晚期发芽 (富含黄类糖化物) 激活PI3K/NQO1.1.
- 在特定阶段对发芽麦进行有针对性的加工,可以根据不同的基签名和机制优化功能性食品的开发.
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