绿色提取采用天然的深溶性溶剂,用于从向日粉中恢复蛋白质
Gulsah Karabulut1, Ilayda Sanli2, Gulay Ozkan2
1Department of Food Engineering, Faculty of Engineering, Sakarya University, Sakarya 54187, Türkiye.
International journal of biological macromolecules
|September 12, 2025
概括
与传统方法相比,绿色提取采用天然深度环氧化溶剂 (DES),如胆:糖 (ChCl:Gly) 改善了向日蛋白的功能和结构保存.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 绿色化学 绿色化学
背景情况:
- 太阳花粉 (SM) 是一种有价值的植物蛋白质来源.
- 传统的性提取方法会导致蛋白质变质,并降低功能.
- 开发可持续的提取技术对于植物性蛋白质至关重要.
研究的目的:
- 为了研究天然的深度环氧溶剂 (DES) 用于向日蛋白质的绿色提取.
- 为了比较DES提取的蛋白质与性提取的蛋白质.
- 评估DES对蛋白质产量,结构,可溶性,消化性和生物可访问性的影响.
主要方法:
- 选了十种DES配方,包括胆:甘油 (ChCl:Gly) 和胆:葡萄糖 (ChCl:Glu).
- 超声波辅助提取用于蛋白质分离.
- 分析蛋白质产量,可溶性,结构完整性 (FTIR,CD),消化性和形态.
- 模拟体外消化用于生物可访问性评估.
主要成果:
- ChCl:Gly和ChCl:Glu产生了最高的可溶性蛋白质含量.
- Cl:Gly分离物显示出优越的溶解性和水解度.
- 与性提取的蛋白质相比,DES提取的蛋白质保留了更好的结构完整性和二次结构 (β转变).
- ChCl:Gly产生的稳定,球形蛋白质颗粒.
- 蛋白质的生物可访问性在所有分离物中都是可比的 (~70%).
结论:
- 天然的深溶性溶剂,特别是ChCl:Gly,为日蛋白提取提供了可持续和高效的替代品.
- 基于DES的提取增强了蛋白质的功能,并保持了结构完整性.
- 这种绿色方法克服了与传统性提取方法相关的局限性.
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