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优化13C稳定同位素标签,用于小鼠模型中研究三糖循环中间体

Jarrod Laro¹, Monica Ness¹, Joseane Godinho²

¹Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK 73019, USA; Department of Chemistry and Biochemistry, San Diego State University, San Diego, CA 92182, USA.

Methods (San Diego, Calif.)

|September 12, 2025

概括

优化了小鼠的13C-葡萄糖玻尿酸标签,这项研究发现4mg/g剂量与90分钟的纳入期产生了最好的三碳酸 (TCA) 循环标签. 禁食的持续时间需要器官特异性调整,以进行准确的代谢研究.

科学领域:

代谢研究的研究.
生物化学生物化学
动物模型动物模型

背景情况:

三碳酸 (TCA) 循环对于能源生产至关重要,并且经常受到疾病的影响.
监测由于疾病导致的TCA代谢变化需要高效和成本效益的方法.
基于13C的稳定同位素标签对于追踪体内代谢变化很有价值.

研究的目的:

优化基于玻尿酸的13C稳定同位素标签,用于研究小鼠的TCA循环.
为了确定最佳参数,包括剂量,服用时间,禁食,前体和TCA标签的途径.
为设计未来的体内TCA循环研究提供指导.

主要方法:

基于玻尿酸的13C稳定同位素标记实验在小鼠模型中进行.
测试了各种参数:13C-葡萄糖剂量 (4毫克/克),服用时间 (90分钟),禁食时间 (0-3小时) 和腹腔内注射路径.
在食道,心脏,脏,肝脏,血和靠近结肠中评估了TCA标签.

主要成果:

通过腹腔内注射的13C-葡萄糖4mg/g剂量,90分钟的内置期提供了最佳的TCA标签.
3小时的禁食改善了大多数器官的标记,除了心脏,它在没有禁食的情况下显示出更好的标记.
与对照组相比,玻尿酸葡萄糖的使用对整体新陈代谢的影响很小.

结论:

这项研究建立了玻尿酸13C-葡萄糖标签的最佳条件,以研究小鼠的TCA代谢.
对于准确的TCA标签,必须对禁食协议进行器官特定的优化.
这些发现对于在疾病模型中推进TCA周期变化的体内研究至关重要.

关键词:

波卢斯 (Bolus) 是一种可怕的东西.碳-13是什么意思禁食禁食是一種禁食.葡萄糖是一种葡萄糖.标签标签标签标签我们的老鼠是老鼠.在TCA循环中,TCA循环是

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