相关实验视频

Updated: Jan 18, 2026

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

通过多重积累前体质谱法改善蛋白质动态范围

Teeradon Phlairaharn^1,2, Ariana E Shannon¹, Xinlei Zeng³

¹Department of Quantitative Health Sciences, Mayo Clinic, Rochester, Minnesota 55905, United States.

Journal of proteome research

|September 12, 2025

概括

科学家们开发了多重积累前体质谱法 (MAP-MS),以提高轨道探测器质谱仪的性能. 这种方法几乎可以在没有硬件更改的情况下将动态范围增加一倍,在数据独立采集中增强类检测.

科学领域:

分析化学分析化学
质谱测量质量谱测量
蛋白质组学是指蛋白质组学.

背景情况:

基于Orbitrap (OT) 的质谱仪对于高分辨率分析至关重要,但与其他质量分析仪相比,其扫描速度较慢.
扩大前体离子检测的动态范围对于质谱学的全面分析至关重要.
目前的方法通常需要复杂的软件或硬件修改来提高性能.

研究的目的:

为了提高Orbitrap质谱仪的前体动态范围,而不需要硬件或软件修改.
引入和评估一种名为多重积累前体质谱法 (MAP-MS) 的新方法.
评估MAP-MS对数据依赖采集 (DDA) 和数据独立采集 (DIA) 方法的影响.

主要方法:

利用长轨道飞行器 (OT) 短暂物来扩大前体动态范围.
修改了选择的离子监测方法,将多个前体m/z范围复杂化为单个扫描,称为多重积累前体质谱 (MAP-MS).
采集数据使用数据依赖获取 (DDA) 和数据独立获取 (DIA) 战略进行评估.

主要成果:

MAP-MS实现了前体动态范围的近2倍增加,没有显著的后果或额外的离子积累时间.
在DDA模式下使用MAP-MS时,前体量化得到了改进,测量质量更高.
在DIA模式下体检测在与MAP-MS结合前体和体质谱时增强了多达11%.

结论:

多重积累前体质谱 (MAP-MS) 是一种有效的,无修改的策略,可显著提高前体的动态范围.
MAP-MS提高了DDA中的定量准确性,并提高了DIA中的检测灵敏度,提供了广泛的适用性.
这种方法代表了高分辨率质谱学的宝贵进步,特别是在复杂的生物样本分析中.

关键词:

数据依赖的数据采集.数据独立的获取采集.质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量多重复杂的多重复杂.轨道飞行式质量分析仪酸标识优化优化的识别.酸量化优化优化酸量化优化

更多相关视频

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

Published on: August 6, 2018

相关概念视频

Tandem Mass Spectrometry

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

Mass Analyzers: Overview

Mass Analyzers: Overview

The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...