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外聚糖化物 (EPS) - - 产生热球菌:功能和益生菌潜力

Dominika Jurášková¹, Vanessa C Pires¹, Susana C Ribeiro¹

¹Institute of Agricultural and Environmental Research and Technology (IITAA), University of the Azores, 9700-042 Angra do Heroísmo, Portugal.

Foods (Basel, Switzerland)

|September 13, 2025

概括

鉴定了山羊奶Streptococcus thermophilus菌株的特征,其中GM4显示出益生菌潜力. 它的外聚糖 (EPS) 显示出抗氧化和降胆固醇的特性,使其适合功能性食品.

科学领域:

微生物学微生物学
食品科学食品科学食品科学
生物化学生物化学

背景情况:

热菌 (Streptococcus thermophilus) 是乳制品发酵中的一个关键的起始培养物.
由S. thermophilus产生的外聚糖 (EPS) 可以为食品提供有益的功能性质.
来自诸如山羊奶等多种来源的EPS生产菌株的特征对于识别新功能成分至关重要.

研究的目的:

从山羊奶中分离出来的产生外聚糖 (EPS) 的Streptococcus thermophilus菌株的特征.
评估生产的EPS的结构和功能特性.
评估所选的S. thermophilus菌株的技术和益生菌潜力.

主要方法:

从山羊奶中分离和描述S. thermophilus菌株.
发酵分析,碳水化合物利用概况和酶活性测定 (例如β-galactosidase,aminopeptidases,酸酸酶).
益生菌潜力的评估:抗生素敏感性,抗氧化能力,降胆固醇能力,自我聚合和与病原体的共同聚合.
通过EPS净化,进行结构分析 (组成,糖链接) 和功能测定 (抗氧化活性).

主要成果:

隔离了四种S. thermophilus菌株,它们表现出高效的乳糖发酵和理想的酶活性.
菌株GM4表现出显著的益生菌特征:高β-银酶活性,中等的抗氧化和降胆固醇能力,以及强大的聚合性质.
转基因4产生了一种抗脱酶的EPS,主要由葡萄糖和曼诺酸组成,具有显著的抗氧化活性.
该EPS结构包括特定的甘氨酸链接,有助于其功能性质.

结论:

菌株 GM4 的 Streptococcus thermophilus 是益生菌和功能性食品应用的一个有希望的候选者.
由菌株GM4产生的EPS具有显著的抗氧化特性,适合纳入功能性食品.
从山羊奶中产生EPS产生的S. thermophilus的表征产生了具有宝贵技术和健康促进属性的菌株.

关键词:

在EPS的EPS系统中.S. 热类动物热类动物其他类型的外聚糖类.乳酸细菌是一种乳酸细菌.益生菌是一种益生菌.

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