在不同的pH值下,从大米白蛋白中获得的加热诱导的粉状纤维的形成和结构特征
1School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China.
Foods (Basel, Switzerland)
|September 13, 2025
概括
中性pH条件促进大米白蛋白 (RA) 纤维化,形成类似虫的纤维. 酸性条件 (pH2) 导致由于水解而形成球形聚合物,阻碍纤维的形成.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
背景情况:
- 米白蛋白 (RA) 是大米中主要的蛋白质.
- 蛋白质纤维化受环境条件的影响,如pH值和温度.
- 了解纤维细胞的形成对于食品产品的开发至关重要.
研究的目的:
- 研究在中性 (pH7) 和酸性 (pH2) 条件下进行热处理对大米白蛋白 (RA) 纤维化的影响.
- 在不同的pH条件下比较RA的结构变化和纤维状形成.
- 为食品应用提供关于优化RA纤维素形成的见解.
主要方法:
- 在pH 7和pH 2下对大米白蛋白 (RA) 进行热处理.
- 测量 thioflavin T (ThT) 强度以评估纤维的形成.
- 使用显微镜分析粒子大小和结构重新排列.
- 评估二次结构变化,特别是β片含量.
主要成果:
- 在中性pH (pH7) 时,RA纤维化在1-4%的蛋白度下增加了27-35%的ThT强度,形成了具有增强β片结构的状纤维 (100-300 nm).
- 在酸性pH (pH2) 时,RA显示ThT强度下降,由于过度水解,溶解性低,水性高,形成大型球形聚合物 (>2000nm).
- 在中性pH下观察到结构重组和粒子大小增加.
结论:
- 中性pH的环境是最佳的白蛋白 (RA) 纤维的形成.
- 酸性条件不利于RA纤维的形成,导致聚合.
- 这些发现为通过受控纤维化在食品中利用RA提供了宝贵的见解.
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