相关实验视频

Updated: Jan 18, 2026

Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations

Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations

Published on: May 5, 2021

在白腿,Litopenaeus vannamei中是一个负增长调节剂

Panpan Niu^1,2,3, Shanshan Jiang^2,3, Mianyu Liu^1,2,3

¹State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.

Animals : an open access journal from MDPI

|September 13, 2025

概括

太平洋白基因LvSlc12A2通过影响中的透平衡和与LvMstn和LvMIH等生长抑制剂相互作用来负面调节生长.

科学领域:

水产养殖是水产养殖的一种方式.
分子生物学分子生物学
生理学生理学

背景情况:

太平洋白 (Litopenaeus vannamei) 对水产养殖至关重要,但生长调节机制尚不清楚.
以前的研究表明,LvSlc12A2在的生长中起作用.
了解LvSlc12A2的功能对于优化养殖至关重要.

研究的目的:

研究LvSlc12A2在Litopenaeus vannamei.的生长调节中的作用.
为了确定LvSlc12A2,透平衡和与生长相关的基因之间的关系.

主要方法:

用RT-qPCR分析基因表达模式.
RNA干扰 (RNAi) 和基因过度表达以研究基因功能.
组织在位杂交以定位基因表达.

主要成果:

LvSlc12A2在上表达最高,其次是肝.
过度表达LvSlc12A2降低了水素 (LvAqp) 的表达,而敲击则增加了它.
在LvSlc12A2的过度表达上调增长抑制剂 (LvMstn,LvMIH),而在Knockdown下调它们.

结论:

LvSlc12A2负面调节的生长,可能是通过与LvMstn和LvMIH的协同作用.
LvSlc12A2通过调节组织中透平衡来影响生长.
这项研究提供了对生长调节的分子机制的见解.

关键词:

利托佩内亚斯 (Litopenaeus vannamei) 是一个水族群.标签: 美国 LvSlc12A2 这是一种RNA干扰.在现场混合化.过度表达是一种过度表达.

更多相关视频

Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine PEI

Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine PEI

Published on: May 24, 2022

Light-Controlled Fermentations for Microbial Chemical and Protein Production

Light-Controlled Fermentations for Microbial Chemical and Protein Production

Published on: March 22, 2022

相关概念视频

Stringent Response in E. coli

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...