猪相关的卵巢转录组分析揭示了microRNA-mRNA调控网络
Jiaxin Li1,2,3, Kai Wang1,2,3, Jianfeng Ma1,2,3
1Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.
Animals : an open access journal from MDPI
|September 13, 2025
概括
猪的子宫内生长限制 (IUGR) 通过破坏细胞循环和亡,损害丸的发育. 这项研究确定了关键的microRNA和相关基因,为IUGR小猪的男性生殖健康提供了洞察力.
科学领域:
- 生殖生物学 生殖生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 动物科学动物科学
背景情况:
- 宫内生长限制 (IUGR) 对畜牧业的发展和经济效率产生负面影响.
- 微RNAs (miRNAs) 在表观遗传调节动物生长和发育方面至关重要.
研究的目的:
- 在IUGR猪的丸中研究miRNA-mRNA调节网络.
- 为了确定IUGR诱导的丸异常的潜在分子标志物.
主要方法:
- 来自IUGR和正常出生体重 (NBW) 猪的丸组织的转录组分析.
- 对信使RNAs (mRNAs) 和miRNAs的差异表达分析.
- 综合分析,构建一个miRNA-mRNA调节网络.
主要成果:
- IUGR猪的丸体积,体重和发育异常都减少了.
- 鉴定了4945个差异表达的mRNA和53个差异表达的miRNA.
- IUGR通过影响细胞周期进展和亡途径来破坏丸发育.
结论:
- 这项研究阐明了在IUGR猪中男性生殖障碍背后的分子机制.
- 特定的miRNAs (例如ssc-miR-23a) 和基因 (例如YWHAZ) 被确定为潜在的生物标志物和治疗点.
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